02 Peripheral Blood Smear and Bone Marrow Hematopoiesis Flashcards Preview

02 Hematology And Immunology > 02 Peripheral Blood Smear and Bone Marrow Hematopoiesis > Flashcards

Flashcards in 02 Peripheral Blood Smear and Bone Marrow Hematopoiesis Deck (50):
1

55%
Pale yellow fluid portion of the whole blood that consists of water and its dissolved content

Plasma

2

Functions of peripheral blood

Transport
Homeostasis
Protection
Good indicators of disease, infection, health

3

Peripheral blood smear stain

Wright stain

4

Basic Wright stain

Nuclei, granules of basophils, RNA of cytoplasm

5

Acidic Wright stain

RBC, granules of eosinophils

6

Erythrocyte diameter

7.8 microns

7

Reticulocyte special staining

Cresyl violet

8

Short conical projections from cell membrane

Echinocytes

9

Causes are low ATP and high osmotic pressure due to hypertonic environment

Crenation

10

Aggregates of RBCs
Usually seen in thick blood smear

Roleaux

11

Roleaux facilitates the ________

Rate of red cell sedimentation

12

Membrane skeleton maintaining biconcave shape

Spectrin
Actin
Ankyrin

13

Forms a lattice reinforcing the membrane

Spectrin

14

For mechanical stability and deformability

Actin and protein bands 4.1 and 4.9

15

Attaches spectrin to plasmalemma
Permits membrane cellular fluidity for passage to capillaries

Ankyrin

16

Presence of RBCs with great size variations

Anisocytosis

17

Presence of RBCs with great shape variations

Poikilocytosis

18

Normal response in living in high altitudes

Polycythemia

19

Platelet peripheral zone
No organelles
High concentration of actin and myosin

Hyalomere

20

Platelet central zone
Contain mitochondria, glycogen, granules
Has canaliculi

Granulomere

21

Platelet granules

Alpha granules
Dense granules

22

Release adhesive proteins, growth modulators and coagulation factors

Alpha granules

23

Initial platelet attachment to blood vessel wall injury

Von Willebrand Factor

24

Secreted by platelets attached to blood vessel wall via vWF
Platelet aggregation

Thrombospondin

25

Stimulates repair of damaged blood vessels

Platelet derived growth factor

26

Counteracts the anticoagulant heparin

Platelet factor IV

27

Harbors ADP, ATP, calcium, polyphosphate and serotonin
Plays a physiologically role in homeostasis

Dense granules

28

Slow cells flowing from the cell venules

P-selectin

29

Migration of leukocytes from leaky channels heading towards the site of injury or infection

Diapedesis

30

Granules present in agranulocyte

Azurophilic granules

31

Contains hypochlorite and other agents toxic to bacteria

Myeloperoxidase

32

Produces holes in microorganism's cell membrane

Defensin

33

Dissolves bacterial cell wall components

Lysozyme

34

First line of defense versus bacteria invasion

Neutrophils

35

Clock face appearance

Plasma cell

36

Principal sues of hemopoiesis

Yolk sac (mesoblastic stage)
Liver and spleen (hepatic phase)
Bone marrow (myeloid phase)

37

2 types of bone marrow

Red (active)
Yellow (inactive)

38

Earliest recognizable stage for RBC lineage
Large nucleus with 2-3 nucleoli
Rim of moderately basophilic cytoplasm

Proerythroblast

39

Nucleus becomes more heterochromatic
More strongly basophilic cytoplasm

Basophilic erythroblast

40

Nucleus with more condense chromatin
Checkerboard appearance or cartwheel chromatin
Large cell capable of division

Polychromatic erythroblast

41

Eccentric nucleus
Pinker cytoplasm with tinge of blue
Dense hetero with little euchromatin
Extruding nucleus

Orthochromatic erythroblast/normoblast

42

Erythropoiesis factors: mitogen for all erythroid progenitor and precursor cells, also promoting their differentiation

Erythropoietin

43

Erythropoiesis factor: proliferation of erythroblasts during differentiation

Vitamin B12 and B9

44

Erythropoiesis factor: hemoglobin synthesis

Iron

45

First stage in lineage of granulocytes
Earliest recognizable stage

Myeloblast

46

Biggest in granulocytopoiesis
Formation of granules

Promyelocyte

47

Rounded nucleus
Specific granules appear
Basophilic Iine can only be seen in this stage
Last stage of cell division

Myelocyte

48

Indented nucleus
Neutrophilic

Metamyelocyte

49

U shaped nucleus

Band

50

Undergoes endomitosis

Thrombocytopoiesis