1-36 FISH, Microarrays and Next Generation Sequencing (NGS) Flashcards Preview

MSI Unit I > 1-36 FISH, Microarrays and Next Generation Sequencing (NGS) > Flashcards

Flashcards in 1-36 FISH, Microarrays and Next Generation Sequencing (NGS) Deck (8)

explain the technique of FISH

flourecence in situ hybridization

DNA probes are labeled wtih flourescent dyes and hybridized to chomrosomes.
-probes made for particular DNA sequences are specific to those sequences and cannot be used for other loci.

the results are observed with flourescent microscope


Fish in clinical setting

used to assay for the presence or absence of a gene of interest whose sequence is known


FISH when testing for a deletion

two different color probes will be used - one for control, and one for gene of interest

someone with no deletion should show four signals

someone with a deletion at the locus of interest should only produce 3
-two control signals
-one signal from chromsome without the deletion


contiguous gene syndromes

Contiguous gene syndromes are diseases that arise from deletions or duplications of regions of DNA that contain genes with generally unrelated functions.


describe microarray analysis

comparison of the quantity of nucleic acid sequence of interest to the quantity of a reference nucleic acid sequence of a known genetic complement


applications of microarray analysis

dna arrays - targets a specific gene of interest in order to genotype a patient

expression (RNA) arrays - targets mRNA sequences to determine which genes are being expressed in a cell

chromosome arrays - assay for chromosome abnormailities


microarrays are not effective in detecting

balances rearrangment, as the amount of DNA between the test and refernce will be the same


genome sequencing determines the

base-pair sequence of the genome.

Decks in MSI Unit I Class (52):