1-36 FISH, Microarrays and Next Generation Sequencing (NGS) Flashcards Preview

MSI Unit I > 1-36 FISH, Microarrays and Next Generation Sequencing (NGS) > Flashcards

Flashcards in 1-36 FISH, Microarrays and Next Generation Sequencing (NGS) Deck (8):
1

explain the technique of FISH

flourecence in situ hybridization

DNA probes are labeled wtih flourescent dyes and hybridized to chomrosomes.
-probes made for particular DNA sequences are specific to those sequences and cannot be used for other loci.

the results are observed with flourescent microscope

2

Fish in clinical setting

used to assay for the presence or absence of a gene of interest whose sequence is known

3

FISH when testing for a deletion

two different color probes will be used - one for control, and one for gene of interest

someone with no deletion should show four signals

someone with a deletion at the locus of interest should only produce 3
-two control signals
-one signal from chromsome without the deletion

4

contiguous gene syndromes

Contiguous gene syndromes are diseases that arise from deletions or duplications of regions of DNA that contain genes with generally unrelated functions.

5

describe microarray analysis

comparison of the quantity of nucleic acid sequence of interest to the quantity of a reference nucleic acid sequence of a known genetic complement

6

applications of microarray analysis

dna arrays - targets a specific gene of interest in order to genotype a patient

expression (RNA) arrays - targets mRNA sequences to determine which genes are being expressed in a cell

chromosome arrays - assay for chromosome abnormailities

7

microarrays are not effective in detecting

balances rearrangment, as the amount of DNA between the test and refernce will be the same

8

genome sequencing determines the

base-pair sequence of the genome.

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