1.1.4 detecting proteins using antibodies Flashcards
1
Q
what are immunoassay techniques used for
A
to detect and identify specific proteins
2
Q
what do immunoassay techniques use to work
A
stocks of antibodies with the same specifity
3
Q
what are monoclonal antibodies
A
those with the same specifity
4
Q
what is an antibody specific to the protein antigen linked to
A
a chemical label; often a reporter enzyme producing a colour change.
5
Q
what types of reporter enzymes are used to link with specific antibodies
A
chemiluminescence
fluroescence
reporter enzyme
6
Q
when is western blotting used
A
after SDS-PAGE electrophoresis
7
Q
how does western blotting work
A
the separated proteins from the gel are transferred onto a solid medium
the proteins can be identified using specific antibodies that have reporter enzymes attached
