11.6.13 Molecular Biology Transcription part 6 Flashcards Preview

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Flashcards in 11.6.13 Molecular Biology Transcription part 6 Deck (36):
1

Required for general cell functions and cell viability.
enzymes of basic metabolism and proteins for cell structures such as ribosomes.
(house keeping genes/regulated genes)

house keeping genes

2

cell-type specific genes which are responsible for the differentiated functions of specific cell types. These also include genes which respond to signals from other cells.
hormone/growth factor/stress responsive genes
(house keeping genes/regulated genes)

regulated genes

3

(prokaryotic/eukaryotes) regulation involves Adjustment of metabolic requirement in response to nutritional environment

prokayrotic

4

T/F e.coli prefers glucose above all other sugars

T

5

cAMP is (high/low) when glucose is present

low

6

DBP carry out (positive/negative) control with CAP-cAMP and (negative/postive) with lac and trp repressor

positive, negative

7

repressors are (negative/positive) control

negative

8

activators are (negative/positive) control

positive

9

class (I, II, III) genese make rRNA

class I

10

class (I, II, III) genese make mRNA, snRNA

class II

11

class (I, II, III) genese make tRNA, snRNA

class III

12

-TATA
-iniatiator
-TATA less and initiator less
are all (promotor/inhibitor)

promotors

13

in the TATA less and initaor less what is there instead for the promoters to recognize?

GC island

14

pol (i. ii) it has silencers that repress transcription

II

15

GTF binds to TATA and INR and positons RNA pol (I, II, III)

II

16

out of all the TFII which one has helicast ATPAase activity ?

TFIIH

17

how many TAFs are there
A)15
b)14
c)13

14

18

TBP is (assymetrical/symmetrical) in its tertiary structure.
Either side of the protein reaches into the (minor/major) grove of the DNA to bind and distort promoter DNA structure.

symmetrical, minor groove

19

whats the structore of activator that binds to a specific DNA sequence?

itsa modular protein

20

How do activator communicate with pol II?

uses co activator

21

what type of motif do homeobox protiens have (helix-turn-helix/helix-loop-helix)

helix-turn-helix

22

name the four types of motifs that DNA binding protein can have

HTH, HLH, ZN finger, leuzine sipper

23

T/F Hox genes control
-morphology of vertebra
-segmentation of hindbrain
-digits of the limbs

T

24

How many human genes are there?
A)25,000
B)30,000
C)40,000

25,000

25

T/F Gene control is dont on a gene-specific or cell specific manner

T

26

Human disease is linked to pol (I, II, III)

II

27

VHL is a ____

genetic disorder

28

VHL binds to subunits (b/C/A/D)

B and C

29

elongin complex (decreases/increases) the rate of trancription elongation

increases

30

T/F Mutations in VHL protein prevent its binding to elongin

T

31

loss of VHL lead to (deactivation/activiation) of angiogneic pathways

activation

32

VHL mutation -> prevents binding of elongin BC--> and thus inhibits the formation of active ubiquitin ligase00> HIF is not degraded --> tumor growth is sustained

T

33

GH stands for ?

growth hormone

34

GH is dependent on binding of trancription factor (VHL/Pit-1)

pit-1

35

How many amino acids are there in Pit-1
A)291
b)391
c)491

291

36

(VHL/Huntington's disease) is an inherited neurodegenerative disease, progressive deterioratoin of motor and cognitive abilities

Huntington's disease