Flashcards in 1.9-PD&CAC Deck (14):
How does pyruvate get from the cytosol to the matrix? (2 membranes)
Through pores in the outer mitochondial membrane, and by pyruvate translocase in the inner membrane
What molecule anti ports with pyruvate into the matrix?
What are the three products of the pyruvate dehydrogenase reaction? Is this reaction reversible?
1. 1 CO2(per pyruvate) 2. 1 NADH (per pyruvate) and 3. 1 Acetyl-CoA (per pyruvate)....THIS RXN IS NOT REVERSIBLE (hence why we can't turn FAT to GLUCOSE )
BIG Q Relating Lecutres!!: What are the 5 prosthetic groups for the Pyruvate Dehydrogenase enzyme complex? hint: 4 water soluble vitamins at work!!
1. TPP (Thiamine PyroPhosphate a B1 Thiamine molecule) 2. Lipoamide 3. FAD (B2 Riboflavin) 4.NAD (B3-Niacin) 5. CoA-SH (B5 PentoThenic Acid)
How many Cs get fed into the CAC? How many Cs come out?
2 in (acetyl-CoA) and 2 out (CO2)
How many NADH and FADH2 are made EACH TURN of CAC? How many CO2?
3 NADH and 1 FADH2...2 CO2
Do we yield ATP out of CAC?
Which high energy molecule do we get out of CAC?
Why does the CAC operate only under aerobic conditions?
Because we need NAD+ and FAD from OxidPhos to be fed into the CAC
AFTER ITS ALL SAID AND DONE (Glycolysis, PD, and CAC) how many CO2s? NADH? FADH2? ATP/GTP? SO theoretically how many ATP out of Aerobic Respiratoin?
CO2: 6, NADH: 10, FADH2: 2, ATP/GTP: 4...Theoretically 32ATP (a BUTT LOAD)
HUGE concept! What are the 4 controls for PD & CAC? Which 3 are regulatory because of their EXERGONIC nature?
1.PD Reaction (most complex) 2. Citrate Synthase 3. Isocitrate Dehydrogenase 4.alpha-ketogluatate dehydrogenase... all the CACs (#s 2,3,4) (2,3,4 are all on R side of the cycle!!)
What are the 4 main allosteric INHIBITORS of PDHComplex?
1. ATP/GTP 2. Acetyl-CoA 3.NADH 4. FAs (all "high energy" molecules)
What are the 4 allosteric ACTIVATORS of the PDHComplex?
1.AMP 2.CoA (=low acetyl-CoA) 3.NAD+ 4.Ca2+ (ODD BALL) (All "low energy" molecules)