27_Steroid hormones & gene regulation Flashcards Preview

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Flashcards in 27_Steroid hormones & gene regulation Deck (15)
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1
Q

Which class of transcription factors have a ligand-binding domain?

A

nuclear hormone receptors

2
Q

What is the function of the trans-activation domain on a TF?

A

recruitment of transcriptional partners/machinery

3
Q

Which TF domain binds to DNA response elements?

A

DNA-binding domain

4
Q

Name some examples of steroid hormone receptors.

A

glucocorticoid receptor, estrogen receptor, thyroid hormone receptor, retinoic acid receptors

5
Q

Where do nuclear hormone receptors bind their ligand?

A

in the cytoplasm

6
Q

What tag is used to mark genes according to parental origin?

A

DNA methylation

7
Q

In DNA methylation, where is the methyl group added?

A

C5 position of cytosine residues

8
Q

Where on the DNA does the GR bind?

A

GRE (glucocorticoid response element)

9
Q

What is the chromatin remodelling complex recruited to acetylated histones? How does it affect chromatin?

A

SWI/SNF

It makes DNA accessible to RNA Pol/ other TF by either sliding the nucleosomes aside OR reorganizing them

10
Q

Which TFs bind to the TATA box?

A

TBP

overall, the TFIID complex… which includes TBP and TAFII250, as well as other TFs

11
Q

What does HDAC do?

A

HDAC = histone deacetylase

12
Q

What is recruited to histones once deacetylated by HDAC?

A

Histone methyltransferases; K9 of H3 is methylated.

13
Q

What are SMRT, N-CoR, and CoREST examples of?

A

transcriptional co-repressors

14
Q

What is Sin3 an example of?

A

HDAC

15
Q

What is SUV39H1 an example of?

A

Histone methyltransferase