2S [LAB]: Serologic Tests (secondary)

Question 1

Principle of TPHA

Answer 1

Indirect Hemagglutination

Question 2

Carrier particles used in TPHA

Answer 2

Red cells (Avian, but Bovine may also be used)

Question 3

Non-virulent strain of Treponema used in TPHA

Answer 3

Reiter

Question 4

Pathogenic strain of Treponema

Answer 4

Nichol

Question 5

In TPHA, the setups are mixed for how long and is left undisturbed for how long?

Answer 5

2 mins
45 mins

Question 6

Principle of VDRL

Answer 6

Flocculation

Question 7

Aggregation of non-cellular particulate matter

Answer 7

Flocculation

Question 8

IgM isotype formed after interaction of phospholipid from spirochetes interacting with immune system of the host

Answer 8

Non-treponemal antibodies

Question 9

Principle of RPR

Answer 9

Flocculation

Question 10

Source of antigen used in RPR

Answer 10

Cardiolipin

Question 11

Alcoholic extract of beef’s heart

Answer 11

Cardiolipin

Question 12

Positive result of RPR

Answer 12

Black aggregates

Question 13

Negative control of VDRL

Answer 13

NSS

Question 14

Rotation condition when doing VDRL

Answer 14

180 rpm for 4 mins

Question 15

Rotation condition when doing RPR

Answer 15

100 rpm for 8 mins

Question 16

Widal test is used in which infection?

Answer 16

Salmonella

Question 17

Weil-Felix test is used in which infection?

Answer 17

Rickettsia

Question 18

Antigen detected in Widal test

Answer 18

Somatic flagellar antigen (from Salmonella)

Question 19

Delayed procedure in Weil-Felix test causes what effect?

Answer 19

Fake agglutination

Question 20

Interpret:

OX-19 (+)
OX-2 (Weak)

Answer 20

Typhus

Question 21

Interpret:

OX-19 (-)
OX-2 (-)
OX-K (+)

Answer 21

Tsutsugamushi fever/ disease

Question 22

In Brucella test, a titer above ___ denotes an active infection

Answer 22

1/80

Question 23

Etiologic agent of syphilis

Answer 23

Treponema pallidum subsp. pallidum

Question 24

Etiologic agent of Yaws

Answer 24

T. pallidum subsp. pertenue

Question 25

Etiologic agent of non-venereal disease

Answer 25

T. pallidum subsp. endemicum

Question 26

Motility of T. pallidum

Answer 26

Corkscrew

Question 27

Also known as the "great imitator", French disease, Italian disease

Answer 27

Syphilis

Question 28

Lesions present in primary syphilis

Answer 28

Hard chancre

Question 29

Body parts affected by the chancre

Answer 29

Glans penis (male) Labia (female)

Question 30

Lesions present n secondary syphilis

Answer 30

Condylomata lata

Question 31

Phase in syphilis infection mistaken as recovery

Answer 31

Latent phase

Question 32

Degeneration of lower spinal cord that is a common manifestation of tertiary syphilis

Answer 32

Tabes dorsalis

Question 33

Lesions present in tertiary syphilis

Answer 33

Gummas

Question 34

Microscope used in the direct detection of syphilis

Answer 34

Dark field

Question 35

The direct method of detection of syphilis is only advisable for which phase?

Answer 35

Primary/ secondary

Question 36

Phospholipid released that will react to the immune system of the host following tissue destruction

Answer 36

Reagin

Question 37

The main antigen in VDRL

Answer 37

Cardiolipin

Question 38

Reagent in VDRL that enhances reagin reactivity

Answer 38

Cholesterol

Question 39

Reagent in VDRL that removes anti-complementary substances of cardiolipin

Answer 39

Lecithin

Question 40

Qualitative serum VDRL uses what size of needle? How much of the Ag suspension per mL will be delivered?

Answer 40

18 g 60 drops

Question 41

Quantitative serum VDRL uses what size of needle? How much of the Ag suspension per mL will be delivered?

Answer 41

19 g 75 drops

Question 42

Conditions of the water bath used in VDRL

Answer 42

56C for 30 mins

Question 43

VDRL is done at what temperature?

Answer 43

23-29 C

Question 44

The VDRL result is observed under what magnification?

Answer 44

LPO

Question 45

RPR reagent that makes the reaction more visible

Answer 45

Charcoal

Question 46

RPR reagent that prevents lipid peroxidation

Answer 46

EDTA

Question 47

RPR reagent that preserves the suspension

Answer 47

Thimerosal

Question 48

RPR reagent that acts as a chemical inactivator

Answer 48

Choline chloride

Question 49

Source of live treponeme antigens used in the T. pallidum immobilization test (TPI)

Answer 49

Rabbit testes

Question 50

Causative agent of Lyme disease

Answer 50

Borrelia burgdorferi

Question 51

Titer that is usually considered as positive for lyme disease screening

Answer 51

1:256

Question 52

Antigen used in TPHA

Answer 52

Avian (turkey) RBCs fixed w/ glutaraldehyde and tannic acid

Question 53

The control cell in TPHA are coated with ___

Answer 53

Reiter's strain

Question 54

The test cell in TPHA are coated with ___

Answer 54

Nichol's strain

Question 55

The Ag suspension in VDRL appears to be ___

Answer 55

Milky white