Flashcards in  Microbiology of Selected Bacteria and Viruses Deck (20):
Humans are what kind of hosts to Arbovirus?
What are the Arboviruses with major impact in the Philippines?
Arboviruses with major impact in the Philippines EXCEPT:
A. Chikungunya virus
B. Dengue viruses
C. Japanese B virus
D. Yellow fever virus
C? Not sure.
Where does replication occur in a Flavivirus
Vector of Dengue Fever
Key pathological feature of dengue hemorrhagic fever
Increased vascular permeability with plasma leakage into interstitial spaces associated with increased levels of vasoactive cytokines
When do you take the 1st serum sample in cases of dengue?
When do you take the 2nd sample?
1st: Onset of disease ASAP
2nd: 2-3 weeks after
P: Leptospira interrogans
F: Leptospira biflexia
How do you make cultures selective for Leptospira?
Addition of neomycin or 5-fluorouracil
True of leptospires :
a. Uses long chain fatty acids as source of energy
b. Organic matter for nitrogen source
Leptospires oxidize long-chain fatty acids as an energy source but use Ammonium salts as a nitrogen source, not organic matter
First sample you get from a patient with Leptospirosis
After 7-10 days what sample should you get from a patient with Leptospirosis?
Gold Standard Serologic Test for Leptospira
Microscope Agglutination Test
Benefits and Drawbacks of One-point Microcapsule Agglutination Test
B: Simple and quick to use
D: Cannot identify serovars
In a LEPTO Dipstick what antibodies are being detected?
1. Oral Doxycycline