7.3.2 Polymerase Chain Reaction: DNA Amplification Flashcards Preview

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Flashcards in 7.3.2 Polymerase Chain Reaction: DNA Amplification Deck (5)
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1
Q

During PCR, DNA is denatured by __________, while the binding of primers is encouraged by __________.

A
  • heating, cooling
2
Q

If PCR was started with a single piece of double-stranded DNA, how many copies of the DNA fragment would there be after 3 cycles?

A
  • 8
3
Q

Which of the following is not true about PCR?

A
  • It requires reverse transcriptase
4
Q

PCR requires which starting materials?

i. DNA to be copied
ii. DNA polymerase
iii. Nucleotides
iv. DNA primers

A
  • i, ii, iii, iv
5
Q

Polymerase chain reaction (PCR)

A
  • is a method of making copies of a specific segment of DNA from a single piece of double-stranded DNA.
  • is a method of making copies of a specific segment of DNA from a single piece of single-stranded DNA.

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