Flashcards in 7.3.2 Polymerase Chain Reaction: DNA Amplification Deck (5)
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1
Q
During PCR, DNA is denatured by __________, while the binding of primers is encouraged by __________.
A
- heating, cooling
2
Q
If PCR was started with a single piece of double-stranded DNA, how many copies of the DNA fragment would there be after 3 cycles?
A
- 8
3
Q
Which of the following is not true about PCR?
A
- It requires reverse transcriptase
4
Q
PCR requires which starting materials?
i. DNA to be copied
ii. DNA polymerase
iii. Nucleotides
iv. DNA primers
A
- i, ii, iii, iv
5
Q
Polymerase chain reaction (PCR)
A
- is a method of making copies of a specific segment of DNA from a single piece of double-stranded DNA.
- is a method of making copies of a specific segment of DNA from a single piece of single-stranded DNA.