Where does TCA cycle occur?
Mito matrix
What enz cat link reaction?
Py dehy
What does FAD stand for?
Flavin adenine dinucleotide
How much ATP, ADP + Pi is gen from NADH?
2.5
Which 3 enz in TCA cycle irrev and why?
citrate synthetase
isocitrate dehydrogenase
ketoglutarate dehydrogenase – to drive cycle in 1 direction
Write the equation for the activation of acetate by CoA
Acetate + CoA –> A CoA + H2O
What is the equation for the link reaction?
Py + NAD+ + CoA –> A CoA + CO2 + NADH + H+
What drives the cycle?
Build up of oxaloacetate
How is rate of TCA cycle reg?
by feedback inhibition of key enzymes
How can glucose be made directly from TCA cycle?
Oxal –> phosphoenol py –> glucose by PEP carboxylase
What is the structure of CoA?
ADP, Pantoic acid, β-alanine, cysteamine
Which other cofactors are required for LR?
thiamine pyrophosphate, lipoic acid, FAD, Coenzyme A
Why is CO2 prod in LR?
Going from py (3c) to A CoA (2C)