ALBUMIN-PROTHROMBIN TEST

Question 1

• Part of the total protein and the most abundant type
• Concentration is directly proportional to the severity of hepatic disease

Answer 1

ALBUMIN

Question 2

The liver synthesized _____ g/day of albumin

Answer 2

9-12

Question 3

Low albumin is seen due to

Answer 3

decreased synthesis

Question 4

ALBUMIN Reference value:

Answer 4

3.5-5.0 g/dL

Question 5

Globulins are precipitated which removes globulin from total protein

Answer 5

Salt Precipitation

Question 6

In Salt Precipitation, The remaining albumin from the total protein is quantitated using _______ _________

Answer 6

biuret reaction

Question 7

Formula of Salt Precipitation

Answer 7

Total protein = Albumin + Globulin

Question 8

T/F. In Salt Precipitation, We precipitate globulin to measure albumin

Answer 8

True

Question 9

Charges protein positively so that the following anionic dyes would bind

Answer 9

Dye Binding

Question 10

most commonly used but a sensitive dye to albumin

Answer 10

Bromcresol Green (BCG)

Question 11

Most specific dye

Answer 11

Bromcresol purple (BCP)

Question 12

Not specific for albumin

Answer 12

Methyl orange

Question 13

more specific to albumin that methyl orange

Answer 13

2-4’-hydroxyazobenzene-benzoic acid (HABA)

Question 14

Dye Binding Interferences:

Answer 14

conjugated bilirubin, aspirin, penicillin, sulfonamides

Question 15

This confirm or check if the globulin is higher than albumin

Answer 15

ALBUMIN/GLOBULIN RATIO

Question 16

seen in patient with cirrhosis, multiple myeloma, macroglobulinemia

Answer 16

Inverted A/G ratio

Question 17

ALBUMIN/GLOBULIN RATIO Reference value:

Answer 17

Reference value: 1.3-3.1g/dL (CF to g/L: 10)

Question 18

ALBUMIN/GLOBULIN RATIO Formula:

Answer 18

𝐴𝐺 𝑟𝑎𝑡𝑖𝑜 =𝐴𝑙𝑏𝑢𝑚𝑖𝑛
𝐺𝑙𝑜𝑏𝑢𝑙𝑖𝑛
Globulin = Total protein – Albumin

Question 19

• Aka Vitamin K Response
• It differentiates intrahepatic disorder from extrahepatic disorder

Answer 19

PROTHROMBIN TEST

Question 20

T/F. In PROTHROMBIN TEST, Administer vitamin K prior to the test intramuscularly (10 mg for up to 1-3 days) because there are factors that are Vitamin K dependent (Prothrombin Group)

Answer 20

True

Question 21

PT Prolonged after vit. K administration =

Answer 21

Hepatic disorder

Question 22

PT Normal after vit. K administration =

Answer 22

Extrahepatic disorder

Question 23

prolonged prothrombin time signifies massive cellular damage

Answer 23

Acute/Chronic Hepatitis