Analytical techniques Flashcards Preview

The Medicine (Y2) > Analytical techniques > Flashcards

Flashcards in Analytical techniques Deck (10):
1

What does HPLC stand for and what is its use?

High performance (pressure) liquid chromatography.
It is used as a method of analysis for the quantification of drugs

2

What is on the x and y axis of HPLC graph?

y - absorbance
x - time

3

How is separation achieved in HPLC?

normal phase
reverse phase

4

HPLC involves mobile phase flowing over stationary phase in a column. True or false?

True

5

What is the stationary phase in HPLC?

Fine silica particles

6

What is the column made from in HPLC?

Stainless steel

7

Reverse phase HPLC is more common in pharmaceutical sciences. Why?

Because most drugs are too polar to be separated using normal phase

8

In normal phase HPLC, the stationary phase is polar and the mobile phase is non-polar. True or false?

True

9

In normal phase, least polar analytes are eluted first, followed by increasing polarity. True or false?

True

10

The performance of HPLC can be improved by making the column length longer. True or false?

True