Flashcards in Analytical techniques Deck (10):
What does HPLC stand for and what is its use?
High performance (pressure) liquid chromatography.
It is used as a method of analysis for the quantification of drugs
What is on the x and y axis of HPLC graph?
y - absorbance
x - time
How is separation achieved in HPLC?
HPLC involves mobile phase flowing over stationary phase in a column. True or false?
What is the stationary phase in HPLC?
Fine silica particles
What is the column made from in HPLC?
Reverse phase HPLC is more common in pharmaceutical sciences. Why?
Because most drugs are too polar to be separated using normal phase
In normal phase HPLC, the stationary phase is polar and the mobile phase is non-polar. True or false?
In normal phase, least polar analytes are eluted first, followed by increasing polarity. True or false?