ASFARVIRIDAE

Question 1

Give the virion properties of Asfarviruses.

Answer 1

Enveloped, double-stranded DNA

Question 2

Give the replication site of Asfarviridae.

Answer 2

Primarily in the cytoplasm but nucleus is need thus “nucleocytoplasmic”.

Question 3

It is the only known DNA arbovirus.

Answer 3

African Swine Fever virus

Question 4

ASF virus is stable in what temperature?

Answer 4

4-20 degrees Celcius

Question 5

ASF virus does not persist for months in meat. True or False?

Answer 5

False

Question 6

ASF virus can be destroyed by ?

Answer 6

1. Heating
2. Treating w/ lipid solvents
3. Some disinfectants such as orthophenylnophenol

Question 7

ASF virus is endemic in ?

Answer 7

Sub-saharan Africa

Question 8

Give the clinical signs of ASF virus.

Answer 8

• Severe hemorrhagic disease with high mortality
• Fever (40-42 C)
• Dyspnea
• Vomiting
• Nasal and conjunctival discharge
• Reddening or cyanosis of ears, snout, and body
• Bleeding from nose and anus

Question 9

Give the 2 patterns of transmission ASF.

Answer 9

1. Sylvatic cycle
2. Domestic cycle

Question 10

Describe the sylvatic cycle transmission of ASF virus.

Answer 10

• Replication of virus occurs in soft ticks —> Ornithodoros spp.
• Trans-ovarial and trans-stadial

Question 11

Describe the domestic cycle transmission of ASF virus.

Answer 11

• Infection through consumption of tissue from acutely infected domestic pigs or warthogs
• Ingestion of uncooked meat from infected pigs
• Direct contact —> Oronasal
• Indirect contact: contaminated transport vehicles, fomites, footwear
• Feeding uncooked swill

Question 12

The protein gene used for genotyping ASF virus.

Answer 12

p72/p73

Question 13

Give the diagnostic methods of ASF virus.

Answer 13

1. Virus isolation
2. Detection of antigen e.g. immunofluorescence
3. PCR
4. Hemadsorption
5. ELISA
6. Immunodiffusion

Question 14

Vaccines are available for ASF. True or False?

Answer 14

False