Bacillus & Listeria Flashcards Preview

Microbiology 386 > Bacillus & Listeria > Flashcards

Flashcards in Bacillus & Listeria Deck (41):
1

  1. What is the morphology of bacilli microscopically?
  2. What is the gram stain of bacilli?

  1. Rods
  2. Positive

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2

True or false:

Bacilli have spores.

True

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3

What is this organism?

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Bacillus anthracis

4

What type of hemolysis does Bacilli use?

NONE

5

What does the Bacillus toxin bind to?

Protective antigen

6

What are the 2 incubation areas for Bacillus anthracis?

  1. Consistent alternation between wet and dry
  2. Soil high in nitrogen

7

How does Bacillus anthracis affect cattle?

Septicemia, toxemia, and death

8

How does Bacillus anthracis affect sheep?

Septicemia, toxemia, death

9

How does Bacillus anthracis affect horses?

Colic, edema of the head, neck, & shoulders

10

How does Bacillus anthracis affect swine?

Affects pharyngeal region

11

How does Bacillus anthracis affect dogs and cats?

Affects pharyngeal region

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12

How can Bacillus anthracis affect a human being?

  1. Respiratory infection
  2. Malignant carbuncle
  3. Gastroenteritis 

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13

How did human beings become infected with Bacillus anthracis?

  1. Woolsorting
  2. Making drums/hides

14

Which species of Bacillus is penicillin suseptible?

Anthracis

15

How might you diagnosis Bacillus anthracis?

  1. Ear slice
    • Do an impression smear 
  2. Culture
    • Typical colonies
    • Penicillin suseptibility
  3. PCR

16

  1. What type of immunizing agents are there for animals?
  2. What about for humans?

  1. Modified live
  2. Killed strain

17

  1. How often must you get revaccinated?
  2. When does immunity typically wane?

  1. Anually
  2. Every 6 months

18

What are your drugs of choice in treating Bacillus anthracis in an animal?

  1. Penicillin
  2. Tetracyclin

19

How might an infection with Bacillus cereus be acquired?

Food poisoining

20

What might predispose an individual to a Bacillus subtilis infection?

  1. Heroin use
  2. Immunocomprimised

21

What problems can Listeria monocytogenes cause in a ruminant?

  1. Abortion
  2. CNS disease

22

What is the morphology of Listeria microscopically?

Gram positive flat rod (coccoidal)

23

How many toxins make up Bacillus anthracis?

What are they?

  1. Protective antigen
  2. Edema factor
  3. Lethal factor

24

Which toxins of Bacillus anthracis act as A-subunits?

  1. Edema factor
  2. Lethal factor

25

How does edema factor work in Bacillus anthracis?

Activates adenylate cyclase and interferes with cell function

26

How does the lethal factor work in Bacillus anthracis?

Proteolyticly cleaves the kinase involved in signal transduction in CD4+ T lymphocytes and fibroblasts.  It inhibits IL-2 production and proliferative responses in the CD4+ T cells.  Cleavage of the MAPK kinase in fibroblasts is apparently lethal to those cells.

27

What is the capsule of Bacillus anthracis composed of?

Poly-D-glutamic acid

28

To be affective, what must the Bacillus anthracis vaccine have in it?

Protective antigen

29

  1. Which animals does Listeria monocytogenes affect?
  2. What problems does it cause in each of these species?

  1. Cattle & sheep
  2. CNS problems in cattle and sheep; abortions in sheep

30

What type of hemolysis does Listeria monocytogenes use?

Narrow zone BETA hemolysis

31

List the laboratory results for Listeria monocytogenes in the following tests:

  1. H2S
  2. Catalase
  3. Litmus milk
  4. Motility

  1. H2S = negative
  2. Catalse = positive
  3. Litmus milk = rapid reduction
  4. Motility = at 22EC by peritrichous flagella

32

What is this organism?

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Listeria monocytogenes

33

  1. In what pH does L. monocytogenes replicate?
  2. At which pH should silage be kept because of this?

  1. 5.5 - 9.0
  2. Below 5

34

List ubiquitous places for L. monocytogenes to be found?

  1. Soil
  2. Intestinal tracts (herbivores)
  3. Feces
  4. Silage
  5. Plant material
  6. Milk

35

Explain in detail how L. monocytogenes gets away from humoral immune responses in the body?

  • Organism is ingested by a macrophage
  • Listeriolysin O destructs the phagosome
  • Listeris is released into the cytosol
  • Surrounded by actin-like filaments from the host cell
  • This structure forms a tail
  • Facilitates movement to the cell membrane of macrophage
  • Cell membrane evaginates
  • Organism transfered to another macrophage
  • Being an intracellular parasite, is NOT susceptible to killing by (1) antibody or (2) complement

36

Listerolysin O allows Listeria to be what type of parasite?

Falcultative intracellular parasite

37

What affects does L. monocytogenes have on cattle?

  1. Circling disease
    • Unilateral facial paralysis
    • Fever
    • Blindness
    • Head pressing
    • Death
  2. Abortion
    • Establishes a bacteremia or septicemia and localizes in the placentomes - that causes the death of the fetus

38

What affect does L. monocytogenes have on sheep and goats?

Same as in cattle:

Circling disease

Abortions

39

What affect does L. monocytogenes have on monogastrics / young ruminants?

  1. Septicemia
  2. Focal necrosis of the liver, spleen, LN

40

What might be your drugs of choice in treating L. monocytogenes?

  1. Ampicillin
  2. Tetracyclin
  3. Penicillin

41