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Flashcards in Bacte Lab Deck (144):
1

are there growth of throat culture in EMB?

nopeeeeeeee

2

this converts H2O2 into O2 and H2O

catalase

3

positive result in catalase test

bubbling

4

negative result in catalase test

no bubbling

5

most reliable teset differentiating S. aureus from other staph spp.

coagulase test

6

the only staph pathogenic to human that produces coagulase

S. aureus

7

preferred plasma for coagulase test

rabbit plasma

8

positive in coagulase test shows

white fibrin clots

9

if the slide coagulase test is negative, __________ must be done

tube coagulase

10

bacteria that do not grow on MAC (urine culture)

S. aureus
S. epidermidis
S. saprophyticus
Enterococcus

11

bacteria with pink colonies on MAC (urine culture)

E. coli
K. pneumoniae
Enterobacter

12

bacteria with colorless colonies on MAC (urine culture)

Proteus
Pseudomonas
Citrobacter

13

bacteria with white creamy appearance on CA (urine culture)

S. aureus
S. epidermidis
S. saprophyticus

14

bacteria with greenish colony on CA

Pseudomonas

15

calibration of an inoculating loop

1 microliter or 0.001 mL

16

in urine culture, when is infection indicated

> 1.0 x 10^5

17

in urine culture, when is contamination indicated

bet. 1.0 x 10^3 and 1.0 x 10^5

18

agar that is trypticase soy or nutrient + outdated human blood or defibrinated sheep blood

Blood Agar

19

BA enables detection of

hemolytic patterns

20

difference of CA to BA

CA > lysed blood

21

CA enables growth of _______

fastidious bacteria

22

special nutrient in CA

nicotinamide adenine dinucleotide or V factor

23

action of Phenylethyl alcohol agar

inhibits Gram (-) bacteria

24

special feauture of Mannitol Salt Agar

high level of NaCl

25

who grows in MSA

Staph spp and salt-tolerant Micro and Enterococcus

26

growth of Stapg in MSA shows

yellow colonies

27

indicator in MSA

phenol red

28

color of phenol red at pH 8.4

red

29

color phenol red at pH 6.8

yellow

30

principle of EMB

selects Gram (-)
LF vs NLF

31

LF on EMB shows

purple colonies to green metallic sheen

32

NLF on EMB shows

colorless colonies

33

what is fermented in MSA

mannitol

34

apperance of E. coli on EMB

green metallic sheen

35

appearance of Enterobacter aerogenes on EMB

fish-eye appearance

36

principle of MAC

selects Gram (-)
LF vs NLF

37

LF on MAC shows

pink to red colonies

38

inhibitor in MAC

bile salt

39

aside from bile salt, what inhibits Gram (+) bacteria on MAC

neutral red and crystal violet

40

the only carbohydrate source in MAC

lactose

41

principle of HEA

selects stool pathogens by inhibiting normal flora of GIT

42

what are inhibited by bile salts in HEA

Gram (+) and Gram (-) coliforms

43

indicator in HEA

bromthymol blue

44

sulfur source of HEA

sodium thiosulfate

45

positive for H2S in HEA shows

black centered colonies

46

nonfermenters in HEA produce

green colonies

47

If one, two or three carbo in HEA is fermented, there are

orange colonies

48

in HEA, pathogens appear ______, normal flora apprear _________

green; yellow

49

principle of XLD

like HEA, but differentiates stool pathogens also

50

special feature of XLD

sodium deoxycholate

51

action of sodium deoxycholate

inhibits Gram (+)
inhibits growth of E. coli
inhibits Proteus swarming

52

phenol red indicator becomes _____ in acidic environment

yellow

53

yellow colonies in XLD indicates

fermentation of xylose

54

most Enterobacteriaceae are xylose positive except

Shigella

55

red colonies with black center in XLD means

Salmonella

56

yellow colonies with black center in XLD means

Citrobacter/Proteus

57

color of Shigella in XLD

red colony

58

principle of SSA

differentiates stool pathogens
inhibits normal flora coliforms

59

in SSA, what inhibits negative coliforms

brilliant green
bile salts

60

appearance of lactose fermenters in SSA

pink-red

61

appearance of normal flora coliform in SSA

pink-to-red colonies

62

appearance of Shigella in SSA

colorless with black center

63

appearance of Salmonella in SSA

colorless with black center

64

this is a selective medium for the isolation of Salmonella

Bismuth Sulfite Agar

65

selective ingredients of BSA

bismuth sulfite
brilliant green

66

ingredient in BSA that inhibits Shigella, Gram (+), most lactose fermenting intestinal flora

brilliant green

67

appearance of S. typhi in BSA

black surrounded by metallic sheen

68

appearance of S. gallinarum, S. choleraesuis, S. paratyphy on BSA

light green colonies

69

TCBS means

thiosulfate citrate bile salt sucrose

70

this is an agar differential and selective for Vibrio spp from stool specimens having mixed flora; also

TCBS

71

TCBS includes ____________

sodium citrate
sodium thiosulfate
oxgall

72

aside from ingredients in TCBS, what allows specific grwoth of Vibrio

high pH

73

aside from Vibrio, what may ocassionally grow on TCBS

Aeromonas
Pseudomonas

74

pH indicator of TCBS

bromthymol blue

75

yellow colonies in TCBS indicates

V. cholera
V. alginolyticus

76

blue green colonies in TCBS indicates

V. parahaemolyticus
V. vulnificus

77

those that are yellow in TCBS fermented _______

sucrose

78

action of TSI

identification of Enterobacteriaceae

79

pH indicator of TSI

phenol red

80

H2S indicator of TSI

ferrous sulfate

81

what is fermented in TSI when the results is K/A

glucose only

82

what is fermented in TSI when results is A/A

glucose + lactose/sucrose

83

what is fermented in TSI when result is K/K

none

84

what is indicated when result in TSI has encircled A

gas production

85

Enterobacteriaceae that results to A/AG in TSI

E. coli
E. aerogenes
P. agglomerans
K. pneumoniae

86

Enterobacteriaceae that results to A/AG or K/AG in TSI

C. koseri
S. marcescens
H. alvei

87

Enterobacteriaceae that results to A/A or K/A

Y. enterocolitica
S. sonnei

88

Enterobacteriaceae that results to K/K

Pseudomonas aeruginosa
Alcaligenes

89

Enterobacteriaceae that results to A/AG+ or K/AG+

C. freundii
S. arizonae

90

Enterobacteriaceae that results to K/AG+

P. vulgaris
P. mirabilis
S. paratyphi
S. choleraesuis
E. tarda

91

Enterobacteriaceae that results to K/A

S. dysenteriae
S. flexneri
S. boydii

92

K in TSI result reporting means

alkalinic/red

93

A in TSI result reporting means

acidic

94

used to determine the ability of the oranism to deaminate lysine, decarboxylate lysine and produce H2S

Lysine Iron agar

95

the only deaminase negative in the family Enterobacteriaceae

Proteeae (Proteus, Morganella, Providencia)

96

pH indicator of LIA

bromcresol purple

97

glucose fermentation in LIA leads to __________

yellow butt

98

lysine decarboxylation leads reverts yellow butt to ______

purple

99

LIA result that indicates lysine decarboxylate (+)

K/K

100

LIA result that indicates lysine decarboxylase (-)

K/A

101

LIA result that indicates deaminase (-)

R/A

102

bacteria that results to K/K LIA result

E. coli
E. aerogenes
K. pneumoniae
S. marcescens
H. alvei

103

bacteria that results to K/A LIA result

P. agglomerans
C. koseri
S. dysenteriae
S. flexneri
S. boydii
S. sonnei

104

bacteria that results to R/A LIA result

Proteus
Providencia
Morganella

105

bacteria that results to K/K+ LIA result

S. typhimurium
S. arizonae
S. typhi
S. paratyphi
S. chloerasuis

106

medium for Indol Production test

tryptone broth

107

rgt in Tryptone broth

Ehrlich's or Kovac's

108

Kovac's rgt is

paraaminobenzaldehyde

109

positive result in Indol prod test shows

red ring

110

negative result in Indol prod test shows

yellow/brown ring

111

principle of Indol prod test

tryptophan oxidized to Indol by tryptophanase

112

methyl red test uses ______ medium

MRVP medium

113

rgt in MRVP medium

methyl red indicator

114

+ in MR test shows

red soln

115

- in MR test shows

yellow soln

116

principle of MR test

glucose is fermented to pyruvic acid

117

Voges-Proskauer tests used _______ medium

MRVP

118

rgt in VP test

VP rgt A
VP rgt B

119

VP rgt A is

alpha naphthol in abs. methyl alc

120

VP rgt B is

KOH with creatine

121

+ in VP test shows

pink to red color

122

- in VP test shows

yellow or copper-like color

123

principle of Voges-Proskauer test

glucose is fermented to acetylmethylcarbinol (acetoin) then converted to diacetyl (red)

124

citrate utilization test uses __________

SCA (Simmon CItrate agar)

125

indicator in SCA

bromthymol blue

126

+ in SCA shows

Prussian blue color

127

- in SCA shows

green color

128

principe of Citrate utilization test

citrate > ammonia > ammonium hydroxide

129

urease test uses _________

urea broth

130

indicator in urea broth

phenol red

131

+ in urease test shows

pink-red

132

- in urease test shows

no color change

133

Sulfide indol Motility test uses ______

Sulfide Indol Motility medium

134

rgt of SIM

Ehrlich's or Kovac's

135

ONPG is

ortho-nitrophenyl-beta-D-galactopyranoside

136

method used for water analysis

multiple fermentation tube technic

137

presumtive test in water analysis uses ______ medium

Brilliant Green Bile lactose (BGBL) broth

138

confirmatory test in water analysis uses ______ medium

EMB

139

+ confirmatory test on EMB of water analysis shows

black colonies w/ metallic sheen

140

- confirmatory test on EMB of water analysis shows

no black colonies w/ metallic sheen

141

+ completed test of water analysis shows

gram negative, nonsporeforming bacilli
turbid and bubble/gas space

142

results in water analysis are expressed in

MPN most probable number

143

ano ung mga favorite ni maam tiburcio

Pseudomonas and Serratia

144

saang agar kinukuha ung pangCatalase test

PEA/MSA