BACTE LAB - Biochemical tests Flashcards Preview

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Flashcards in BACTE LAB - Biochemical tests Deck (41):
1

LIA Carbohydrate

glucose

2

amino acid in LIA

Lysine

3

ph indicator

bromcresol purple

4

H2S indicator

ferric ammonium citrate

5

Lysine decarboxylase +

K/K purple over purple

6

Lysine decarboxylase -

K/A purple/yellow

7

Deaminase -

R/A red/yellow

8

H2S positive in LIA

blackening

9

indol production test medium

tryptone broth

10

indol production test negative result

yellow or brown ring

11

indol production test reagent

Ehrlich's or Kovac's (paraaminobenzaldehyde)

12

indol production test + result

red ring

13

methyl red test medium

MRVP or Methyl Red Voges Proskauer medium

14

methyl red test reagent

methyl red indicator

15

methyl red test + result

red solution

16

methyl red test - result

yellow solution

17

voges proskauer test medium

MRVP medium

18

voges proskauer test reagent

VP Reagent A (alpha naphthol in absolute methyl alcohol)
VP Reagent B (KOH w/ creatine)

19

what is produced in the voges proskauer test

acetoin

20

In the VP Test, acetoin is converted to?

diacetyl

21

Citrate utilization test medium

simmon citrate agar

22

Citrate utilization test indicator

bromthymol blue

23

Citrate utilization test + result

prussian blue color

24

Citrate utilization test - result

Green color

25

urease test medium

urea broth

26

urease test indicator

phenol red

27

urease test positive result

pink to red

28

urease test negative result

no color change

29

SIM MEDIUM

Sulfide indol motility medium

30

SIM Reagent

Ehrlich's or Kovac's

31

Containers in water analysis

sterile glass bottles w/ round glass stoppers or screw caps

32

Volume of water in water analysis

not less than 100 ml

33

method in water analysis

multiple fermentation tube technique

34

medium in water analysis

Brilliant Green Bile Lactose Broth

35

tubes used in water analysis

durham
loeffler
hard glass

36

double strength concentration

80 g/L

37

single strength conc

40 g/L

38

positive confirmed test result in water analysis

black colonies w/ metallic sheen on emb

39

negative confirmed test result in water analysis

no black colonies w/ metallic sheen on emb

40

positve completed test result

Gram (-) nonsporeforming bacilli
presence of turbidity
bubble/gas/space

41

the results of the examinations of the replicate tubes and dilutions is expressed in terms of

most probable number (mpn)

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