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1

– materials containing all essential nutrients for bacterial growth

-→ for bacterial growth
-culture media may be in granular or powdered form

Culture media

2

-food and soil (where it is planted) for bacteria

Culture media

3

for how long and at what temperature must the CM have for the bacteria to grow

35 – 37C, 18 – 24 hours

4

2 forms of CM

granular or powdered form

5

CM for motility

Sulfide-Indole-Motility (SIM)

6

Characteristics of Good Culture Media

1. Sterile

2. Free of inhibitory substances

3. Adequate amount of Salt, Water & Certain Essential Nutrients
4. Has a proper pH

7

Classif of CM accdg to physical state or consistency

solid
semi-solid
liquid

8

Classif of CM accdg to physical state or consistency
no solidifying agent

liquid

9

Classif of CM accdg to physical state or consistency
routinely used for bacterial cultivation

liquid

10

sign of bacterial growth in liquid media

turbidity

11

example of liquid media

broth

12

Classif of CM accdg to physical state or consistency
with solidifying agent

solid

13

most common solidifying agent

agar

14

examples of solidifying agents

agar
albumin
gelatin

15

solidifying agent that is used as a diagnostic medium

*Gelatin

16

agar is liquid at what temp

100C

17

agar is solid at what temp

38C

18

Gelatin is solid at what temp

25C

19

agar concentration of solid media

1.5-3%

20

agar conc of semi-solid media

0.5 - 1%

21

Classif of CM accdg to physical state or consistency
used to determine if bacteria is motile or not

semi-solid

22

classif based on application or function

basal/ordinary/simple
enriched
selective
differential
assay medium
maintenance w/o glucose

23

classif based on application or function
useful for assaying vitamins, antibiotics, amino acids

assay medium

24

classif based on application or function
with inhibitory substances

selective

25

classif based on application or function
differentiates types of bacteria using dye or indicator

differential

26

classif based on application or function
supports growth of fastidious bacteria
enriching substances are added

enriched

27

classif based on application or function
basic medium from which you can prepare different media

Simple/Basal/Ordinary

28

classif based on application or function
supports the growth of non-fastidious bacteria

Simple/Basal/Ordinary

29

bacteria that grows immediately in a nutrient agar without added enriching substances

non-fastidious bacteria

30

enriching substances added:

• blood
• serum
• acidic fluid

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