Bacte Lab - Quiz 1 Flashcards Preview

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Flashcards in Bacte Lab - Quiz 1 Deck (48):
1

– materials containing all essential nutrients for bacterial growth

-→ for bacterial growth
-culture media may be in granular or powdered form

Culture media

2

-food and soil (where it is planted) for bacteria

Culture media

3

for how long and at what temperature must the CM have for the bacteria to grow

35 – 37C, 18 – 24 hours

4

2 forms of CM

granular or powdered form

5

CM for motility

Sulfide-Indole-Motility (SIM)

6

Characteristics of Good Culture Media

1. Sterile

2. Free of inhibitory substances

3. Adequate amount of Salt, Water & Certain Essential Nutrients
4. Has a proper pH

7

Classif of CM accdg to physical state or consistency

solid
semi-solid
liquid

8

Classif of CM accdg to physical state or consistency
no solidifying agent

liquid

9

Classif of CM accdg to physical state or consistency
routinely used for bacterial cultivation

liquid

10

sign of bacterial growth in liquid media

turbidity

11

example of liquid media

broth

12

Classif of CM accdg to physical state or consistency
with solidifying agent

solid

13

most common solidifying agent

agar

14

examples of solidifying agents

agar
albumin
gelatin

15

solidifying agent that is used as a diagnostic medium

*Gelatin

16

agar is liquid at what temp

100C

17

agar is solid at what temp

38C

18

Gelatin is solid at what temp

25C

19

agar concentration of solid media

1.5-3%

20

agar conc of semi-solid media

0.5 - 1%

21

Classif of CM accdg to physical state or consistency
used to determine if bacteria is motile or not

semi-solid

22

classif based on application or function

basal/ordinary/simple
enriched
selective
differential
assay medium
maintenance w/o glucose

23

classif based on application or function
useful for assaying vitamins, antibiotics, amino acids

assay medium

24

classif based on application or function
with inhibitory substances

selective

25

classif based on application or function
differentiates types of bacteria using dye or indicator

differential

26

classif based on application or function
supports growth of fastidious bacteria
enriching substances are added

enriched

27

classif based on application or function
basic medium from which you can prepare different media

Simple/Basal/Ordinary

28

classif based on application or function
supports the growth of non-fastidious bacteria

Simple/Basal/Ordinary

29

bacteria that grows immediately in a nutrient agar without added enriching substances

non-fastidious bacteria

30

enriching substances added:

• blood
• serum
• acidic fluid

31

examples of enriched media (3)

• Loeffler’s serum agar
• Chocolate Agar – contains RBCs
• Blood Agar

32

ex of differential media

EMB
MacConkey
Mannitol-Salt agar

33

indicator of Eosin-methylene blue agar

eosin-methylene blue

34

differential media used for for Escherichia coli (greenish metallic sheen)

EMB

35

differentiates lactose fermenters from non-lactose fermenters

EMB

36

colors of lactose fermenters

purple, red, pink

37

colors of non-lactose fermenters

white, colorless, yellow

38

indicator of MacConkey

neutral red

39

indicator of Mannitol-Salt agar

phenol red

40

differential media for growth of Staphylococcus aureus
-

Mannitol-Salt agar

41

differentiates Staphylococcus aureus from Staphylococcus epidermidis

Mannitol-Salt agar

42

selective media that inhibits growth of Gram (+):

• Gentian violet
• Na deoxycholate
• Bile salt

43

selective media that Inhibits growth of Gram (-)

• Na azide
• K tellurite

44

selective media which Prevents spreading of Proteus

• Chloral hydrate
• Alcohol

45

classif accdg to composition

synthetic
non-synthetic

46

classif accdg to composition
exact composition of culture media is known

synthetic

47

classif accdg to composition
exact composition of culture media is not known

non-synthetic

48

classif accdg to form or distribution

plated
tubed

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