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Flashcards in Bacte Lec - Culture Media Lecture Deck (109):
1

bacterial nutritional requirements (3)

water
energy source
carbon source

2

types of organisms based on their source of energy

phototrophs
chemotrophs

3

types of organisms based on their carbon source

heterotroph
autotrophs

4

types of chemotroph

lithotrophs
organotrophs

5

types of organisms based on their carbon source
require CO2

autotrophs

6

types of organisms based on their carbon source
require organic compounds

heterotrophs

7

types of organisms based on their energy source
require light

phototrophs

8

types of organisms based on their energy source
require chemicals

chemotrophs

9

prevention of bacterial growth by water removal

lyophilization

10

organisms which need co2

capnophiles

11

examples of capnophiles

Haemophilus influenzae
Neisseria gonorrhoea

12

energy source: light
C source: CO2

photoautotrophs

13

energy source: light
C source: organic compounds

photoheterotrophs

14

energy source: inorganic compounds
C source: CO2

chemoautotrophs

15

energy source: organic compounds
C source: organic compounds

chemoheterotrophs

16

photoautotrophs
energy source

light

17

photoheterotrophs
energy source

light

18

chemoautotrophs
energy source

inorganic compounds

19

chemoheterotrophs
energy source

organic compounds

20

chemoheterotrophs
c source

organic compounds

21

chemoautotrophs
c source

co2

22

photoheterotrophs
c source

organic compounds

23

photoautotrophs
c source

CO2

24

Representative bacteria of photoautotrophs

cyanobacteria
photosynthetic bacteria
algae

25

Representative bacteria of photoheterotrophs

photosynthetic bacteria

26

Representative bacteria of chemoheterotrophs

protozoa, fungi, bacteria

27

Representative bacteria of chemoautotrophs

sulfur, iron, ammonia oxidizing bacteria
methane producing bacteria

28

essential elements needed by microorganisms [TOM]

Metals
Trace Elements
Organic Growth Factors

29

metals needed by microorganisms

K, Mg, Ca, Fe

30

Trace elements needed by microorganisms

Molybdenum
Copper
Cobalt
Zinc

31

Organic growth factors

purine and pyrimidine
amino acids
vitamins

32

elements of culture media [6]

free of inhibitory substances
proper ph
sufficient moisture
desired consistency
initially sterile
right nutrients

33

classification of CM according to composition

synthetic, chemically defined
non-synthetic, chemically undefined, complex

34

classification of CM according to composition
specific substances can be identified

synthetic, chemically defined

35

examples of synthetic, chemically defined

nutrient agar/broth

36

classification of CM according to composition
specific substances cannot be identified

non-synthetic, chemically undefined, complex

37

ex of non-synthetic, chemically undefined, complex

yeast extract, beef extract

38

digested proteins rich in protens, amino acids, nitrogen and carbon source

peptone

39

form of dehydrated culture media

granular
powder

40

glassware for preparation of plated cm

erlenmeyer flask

41

glassware for preparation of tubed cm

beaker

42

classification of CM according to physical state

solid
liquid
semi-solid

43

agar composition in solid cm

1.5 -2 %

44

agar compostion in semi solid

0.5 - 1%

45

complex polysaccharide [marine algae]

agar

46

temp of liq agar

100c

47

temp of solid agar

40C

48

adv of albumin as solidifying agent

coagulates at high temperature

49

solidifying agent: coagulates at high temperature

albumin

50

solidifying agent: diagnostic medium

gelatin

51

3 solidifying agent

agar
albumin
gelatin

52

ex of semisolid medium

SIM

53

semisolid medium which determines motility or ability to produce hydrogen sulfide and also for indole detection

Sulfide Indole Motility

54

agar composition of liquid medium

0%

55

merely a solidifying agent

agar agar

56

if you are to prepare 100 ml of a certain culture media, how much in g of agar agar should you add

1.5 - 2g

57

classif of cm according to function

>general isolation/supportive/simple/basal/ordinary
>enriched/nonselective
>selective
>differential
>enrichment

58

classif of cm according to function
non-fastidious bacteria

general isolation/supportive/simple/basal/ordinary

59

classif of cm according to function
fastidious bacteria

enriched/nonselective

60

nutrient supplements placed in an enriched or nonselective culture medium

blood
ascitic fluid
serum

61

difference between enriched and enrichment

enriched - promotes growth of all organisms
enrichment - promotes growth of organism of interest

62

difference between CAP and BAP

BAP - blood is added when the basal medium has cooled
CAP - blood is added when the basal medium is hot, which causes lysis of cells, attributed to the chocolate color

63

temp when to added Blood in nutrient agar

40-45C

64

basal medium of bap and cap

nutrient agar

65

types of blood [3]

5% defibrinated sheep's blood
horse blood
outdated human blood

66

types of blood
for fastidious like Haemophilus

5% defibrinated sheep's blood

67

types of blood
for differentiation of various types of hemolysis

5% defibrinated sheep's blood

68

Media with solid and liquid

biphasic

69

type of hemolysis
no hemolysis

gamma hemolysis

70

type of hemolysis
complete hemolysis

beta hemolysis

71

type of hemolysis
partial hemolysis

alpha hemolysis

72

type of hemolysis
greenish discoloration that surrounds a bacterial colony

alpha hemolysis

73

___ hemolysis is characteristic of Streptococcus pyogenes and some strains ofStaphylococcus aureus.

beta

74

___ hemolysis is characteristic of Streptococcus pneumonia

alpha

75

type of blood
gives erratic or consistent results

horse blood

76

disadv of outdated human blood

contains: antibacterial substances, excess citrate ions, other substances (antibodies)

77

agar which contains heated blood or enzyme treated blood

chocolate agar

78

CAP contains what type of blood

heated blood or enzyme treated blood

79

anticoagualant for blood

citrate phosphate dextrose [CPD] or CPD A

80

prevents clotting

defibrination

81

what to place in an EF to prevent clotting/defibrination

paper clips or marbles [fibrin will adhere to these]

82

classif of cm according to function
distinguishes between organisms based on appearance on the medium

differential medium

83

classif of cm according to function
distinguishes between lactose fermenters and non-lactose fermenters

differential medium

84

classif of cm according to function
contains indicators

differential medium

85

classif of cm according to function
enhances bacterial growth of desired bacteria

selective medium

86

inhibitory substances in selective medium

bile salts
sodium azide
sodium desoxycholate
potassium tellurite
gentian violet
antibiotics

87

blood agar can be enriched and differential

enriched: addition of blood
differential: differentiates hemolytic reactions

88

classif of cm according to function
tinsdale agar

selective

89

classif of cm according to function
MSA

selective

90

classif of cm according to function
SSA

selective

91

classif of cm according to function
LJ

selective

92

Components of transport medium

buffer and salts

93

special cm
maintain viability w/o altering concentration

transport medium

94

special cm
temporary storage of chemical sx

transport medium

95

special culture medium
enhances growth of bacteria that may be overgrown by competitors

enrichment broth

96

special cm
layers of living cells

cell line/tissue culture

97

special cm
series of battery test medium

biochemical test media

98

examples of biochemical test media [3]

enterotube
API Test OR API Medium
Packaged Test System

99

series of color reactions matched with a color chart with an interpretation

biochemical test media

100

added when an organism is suspected to be an anaerobe

mineral oil

101

color of nonfermenter

red

102

color of fermenter

yellow

103

displacement of agar interpretation

gas production capability of organism

104

blackening of butt interpretation

H2S production

105

small tubes used to detect gas production

Durham tube

106

growth on the bottom of thioglycollate broth

anaerobe

107

growth on the surface of thioglycollate broth

aerobe

108

growth on the middleof thioglycollate broth

microaerophilic

109

indicates that organism is toxic

cytopathic effect (CPE) or cell death

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