Bacte Lec - Entero Part 2 Flashcards Preview

► Med Misc 05 > Bacte Lec - Entero Part 2 > Flashcards

Flashcards in Bacte Lec - Entero Part 2 Deck (49):
1

LIA: Lysine decarboxylase +

Purple/purple

2

LIA: Lysine decarboxylase -

Purple/yellow

3

LIA: Deaminase positive

Red/Yellow

4

LIA: Blackening

H2S positive

5

IMViC

Indole Methyl red voges proskauer citrate test

6

tryptone is present in what cm

tryptone broth

7

when tryptone is utilized, what is the by product?

indole

8

+ result in indole tests

red ring when overlayed with Kovac’s or Ehrlich’s reagent

9

what is the Kovac’s or Ehrlich’s reagent

PDAB (Para-diethylaminobenzaldehyde or Para-dimethylaminobenzaldehyde)

10

if tryptone broth is not available, what to use?

SIM

11

enzyme in indole tests

tryptophanase

12

- result in indole tests

yellow or brown ring [no tryptophanase]

13

glucose by products

acetoin
pyruvic acid

14

meaning of K/K on LIA

decarboxylation (purple/purple)

15

meaning of R/A on LIA

deamination (red/yellow)

16

when acetoin is oxidized by air or KOH, it is transformed to

diacetyl

17

diacetyl in MR test is determined by adding

VP reagent

18

what is the VP Reagent

5% alpha naphthol in absolute methyl alcohol
40% KOH w/ 0.3% creatine

19

+ result in VP test

pink-red color [unstable]

20

MR & VP cannot be both [what result + or -]

cannot be both +

21

color of methyl red in acidic solutions

red

22

mixed acid fermentation makes the medium

acidic, pH less than 4.4

23

pH range of methyl re

4-6

24

+ result in MR test

red color

25

culture medium of citrate utilizaton test

simmon citrate agar

26

principle of citrate utilizaton test

based on degradation of citrate

27

indicator of sca

bromthymol blue

28

+ result in citrate utilizaton test

blue [means citrate is degraded]

29

- result in citrate utilizaton test

green

30

rep organism for gram (-)

Escherichia coli

31

rapid urease producing organisms

Proteus, Campylobacter, H. pylori

32

best way to differentiate enterobacter and klebsiella

motility test

33

best motility test

hanging drop

34

identify organism
LF
GMS on emb
IMViC Test: ++--

escherichia

35

identify organism
LF
dark purple on emb
IMViC Test: --++

enterobacter
klebsiella

36

less mucoid
urease negative
motile
w/o capsule
center [fish eye]

enterobacter

37

mucoid more
urease +
nonmotile
w/ capsule

klebsiella

38

nonlactose fermenter, spreading/swarming

Pseudomonas
Proteus/Morganella

39

nonlactose fermenter, spreading/swarming, pigmented,

pseudomonas

40

reaction of pseudomonas in TSI

K/K

41

nonlactose fermenter, spreading/swarming, non-pigmented,

Proteus
Morganella

42

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A

Morganella morganii

43

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +

P. vulgaris or P. mirabilis

44

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol +

P. vulgaris

45

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol -

P. mirabilis

46

odor of proteus

burned gunpowder

47

odor of pseudomonas

fruity

48

+ result in urease test

red color

49

- result in urease test

salmon/yellow color

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