BACTE LEC - Entero Part 3 Flashcards Preview

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Flashcards in BACTE LEC - Entero Part 3 Deck (85):
1

principle of phenylalanine test

Deamination of phenylalanine to phenylpyruvic acid

2

positive rxn of the phenylalanine test

Positive (green color in the syneresis fluid

3

ONPG meaning

O-nitrophenyl-beta-D- galactopyranoside

4

test for Slow or late lactose fermenter

O-nitrophenyl-beta-D-galactopyranoside

5

only one that is ONPG positive
Salmonella serotype

Salmonella arizonae

6

rxn of citrobacter in ONPG

+

7

E. coli is a normal flora of the

GI tract

8

Closely related to Shigella (DNA homology)

Escherichia coli

9

“Montezuma’s revenge” “turista” Traveller’s diarrhea

Enterotoxigenic E. coli (ETEC)

10

diseases caused by ETEC

“Montezuma’s revenge”
“turista”
Traveller’s diarrhea

11

E. coli strain
Severe epidemic diarrhea = cholera Fever, headache, vomiting, chills

Enterotoxigenic E. coli (ETEC)

12

E. coli strain
Disease = Shigellosis

Enteroinvasive E. coli (EIEC)

13

Invasion of intestinal epithelium
E. coli strain

Enteroinvasive E. coli (EIEC)

14

E. coli strain
Fever, cramps, bloody stool w/ RBCs, neutrophil and mucus

Enteroinvasive E. coli (EIEC)

15

Verotoxin causes what diarrhea

bloody diarrhea

16

Sorbitol negative SMAC

Enterohemorrhagic E. coli (EHEC)

17

Verotoxic E. coli (VTEC)
0157 H7




Enterohemorrhagic E. coli (EHEC)

18

E. coli strain
Hemolytic uremic syndrome

Enterohemorrhagic E. coli (EHEC)

19

E. coli strain
Hemorrhagic colitis

Enterohemorrhagic E. coli (EHEC)

20

strain number of Enterohemorrhagic E. coli (EHEC)

0157 H7

21

Resembles E. coli
Lactose negative & nonmotile
Shigellosis (bacillary dysentery)

Shigella

22

Shigella: lactose (-) or (+)?

Lactose negative

23

Motility of Shigella

nonmotile

24

disease caused by shigella

shigellosis

25

bacillary dysentery

shigellosis

26

with fishy odor

shigella

27

enteric bacteria which causes Bloody, mucoid, diarrheic stool w/ pus cells & fishy odor

shigella

28

significant shigella species [4]

S. sonnei
S. dysenteriae
S. flexneri
S. boydii

29

Shigella spp
Most prevalent in the US
Neurotoxin & enterotoxin
Non-mannitol fermenter

S. dysenteriae

30

Shigella spp.
Beta galactosidase (ONPG +) ODC +

S. sonnei

31

Motile & ODC Positive
Large amount of gas from glucose

Enterobacter

32

Enterobacter spp
Predominant isolate UTI & wound infection

E. cloacae

33

Enterobacter spp
Resembles E. cloacae
Yellow pigmented Neonatal sepsis

E. sakazakii

34

Short & nonmotile
encapsulated
Large, mucoid colonies
Strings out when touched
by the needle

Klebsiella

35

Klebsiella
Bacterial pneumonias


K. Pneumoniae

36

Kleb spp.
Blood & stool culture Indole positive

K. oxytoca

37

Kleb spp.
Atrophy of the nasal mucous membranes w/ fetid odor
Blood & stool

K. ozanae

38

Kleb spp.
Rhinoscleroma Destructive granuloma of the
nose & pharynx


K. rhinoscleromatis

39

impt spp of klebsiella [poor]

pneumonia
oxytoca
ozanae
rhinoscleromatis

40

impt spp of serratia [morl]

marcescens
liquefaciens
odorifera
rubidaea

41

Opportunistic pathogens
Deoxyribonuclease, lipase, gelatinase
red pigment

serratia

42

serratia spp.
Pneumonia & septicemia

S. marcescens

43

serratia spp.
Rancid, potato-like odor

S. odorifera

44

serratia spp.
Ferment arabinose

S. liquefaciens

45

Negative to Lactose, sucrose, sorbitol Citrate, DNAse, lipase

Hafnia alvei

46

Contaminated IV fluids
Yellow pigment
Negative to ADH, LDH, ODC

Pantoea agglomerans

47

number of serotypes of salmonella

2200

48

very complex genus

salmonella

49

classification of salmonella serotypes

Kauffman - White

50

enteric [typhoid] fever lasts for __

1 week

51

appearance of salmonella on BSA and BGA

Metallic colonies with black ring on bsa

52

sx for disease by salmonella - 1st and 2nd week

blood

53

sx for disease by salmonella - 3rd and 4th week

urine

54

sx for disease by salmonella - indefinite

stool

55

clinical diseases induced by salmonella

septicemia
enteric fever
gastroenteritis

56

antigen test for salmonella

widal test

57

o antigen in widal test meaning

active infection

58

h antigen in widal test meaning

past immunization or infection

59

Salmonella spp w/c causes
Enteric fever
Typhoid & paratyphoid

S. typhi

60

Salmonella spp w/c causes
septicemia

S. choleraesius

61

Salmonella spp w/c causes
gastroenteritis

S. typhimurium
S. enteritidis

62

salmonella test
Amebocytes of horse crab (visible gel)
endotoxin

linulus test

63

Ballooning of the intestinal tract or ileal loop (accumulation of fluid)
enterotoxin

ileal loop test

64

2 genera that citrobacter resembles

salmonella
e. coli

65

2 impt spp of citrobacter

freundii
diversus

66

tribe of Proteus Providencia Morganella

proteeae

67

tribe proteeae
Rapid urease activity “swarming” motility
on BA

proteus

68

Source of GI infection W
ound infections abscesses
Trauma or accidents (aquatic environment)
Biochemically similar to E. coli

Edwardsiella tarda

69

Abundant H2S in TSI agar
Lactose negative
LDC & ODC positive ADH negative

Edwardsiella tarda

70

size/appearance of yersinia on MAC

pinpoint

71

morphology of yersinia

coccobacilli

72

yersinia spp motile at RT

y. pestis

73

genus: nonmotile at 37C

Yersinia

74

yersinia spp which
causes enterocolitis
Ingestion of contaminated water, pork, beef or milk products
Fecal-oral transmission

Y. enterocolitis

75

Ferments mannitol and appears as red, “bull’s eye” colonies w/ colorless halo
CIN medium

yersinia enterolitica

76

types of plague by y. pestis

bubonic, septicemic, pneumonic

77

causes plague [black death]

y. pestis

78

vector of y. pestis

flea [through a bite]

79

yersinia with safety pin appearance

yersinia pestis

80

yersinia
“Stalactite patterns”

yersinia pestis

81

yersinia spp.
ferments both sucrose & sorbitol

yersinia enterolitica

82

yersinia spp.
fails to ferment both sucrose & sorbitol

yersinia pseudotuberculosis

83

Citrobacter species
H2S +
KCN +

Freundii

84

Citrobacter species
Indol +

C diversus

85

Yersinia spp.
Wild animals & game fowl
Lymphadenitis in children
ODC negative Fails to ferment both sucrose & sorbito

Y. pseudotuberculosis

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