BACTE LEC - Misc Gram-negative Bacteria Flashcards Preview

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Flashcards in BACTE LEC - Misc Gram-negative Bacteria Deck (99):
1

Nonmotile
Pleomorphic coccobacilli or rods [2]

Pasteurella multocida
Haemophilus

2

require blood for growth

haemophilus

3

type of factor
provided by blood agar
hematin or hemin
Unknown

X factor

4

type of factor
provided by NAD or coenzyme 1
Vitamin

V factor

5

species of haemophilus w/c require only V factor

H. ducreyi*
H. parainfluenza
H. paraphrohaimolyticus
H. paraphrophilus

6

not adequate to isolate Haemophilus bc it releases NADase

Sheep blood agar

7

inactivates NAD, no NAD, no V factor

NADase (by sheep blood agar)

8

culture medium w/o NADase

horse or rabbit blood agar

9

type of agar
may release hematin and inactivate NADase

choc agar

10

method for the primary isolation of Haemophilus

staph streak

11

in staph streak method, growth near staph is __ [describe]

luxuriant

12

in staph streak, what organism provided nutrients for haemophiilus growth

staph

13

type of hemolysis by s. aureus that releases hematin

beta

14

phenomenon in which there are tiny colonies around staph colony

satellite phenomenon

15

pathogenic spp of Haemophilus
common isolate in CSF if there is meningitis esp among children

H. influenzae

16

VF of H. influenzae

Capsule
IgA protease
Adherence mechanism
Outer membrane components

17

vaccine for influenzae

Hib vaccine

18

clinical manifestations of H. influenzae [3]

meningitis
epiglottitis
bacterial tracheitis

19

Causative agent of acute contagious conjunctivitis

H. aegyptius

20

other name of acute contagious conjunctivitis

pinkeye/sore eye

21

also called Koch-Weeks Bacillus

H. aegyptius

22

general MoT of haemophilus spp except for

inhalation

23

MoT of ducreyi

sexual contact

24

CA of Brazilian purpuric fever (BPF)

H. influenzae biogroup aegyptius

25

causes chancroid or soft chancre

H. ducreyi

26

causes transmitted genital ulcer disease

H. ducreyi

27

causes suppurative, enlarged, draining, inguinal lymph nodes

H. ducreyi

28

sx for H. influenzae

blood, CSF, from upper and lower RT,

29

sx for ducreyi

vaginal swab
abscess drainage

30

sx for aegyptius

swab for conjunctivae

31

atmospheric requirements of haemophilus

5-10% CO2 - Capnophilic

32

microscopic appearance of ducreyi

shoal, school of fish

33

shoal of fish
loosely coiled clusters of organisms lined up in parallel or appearing as fingerprints

H. ducreyi

34

Beta hemolytic on SBA
Clumpy nonhomogenous appearance

ducreyi

35

MAC appearance: larger and more mucoid

ducreyi

36

CAP: translucent, tannish, moist, smooth convex
w/ mousy or bleachlike odor

ducreyi

37

odor of ducreyi on CAP

mousy or bleachlike

38

most misc gram (-) bacilli are __ [atmospheric requirement and location]

obligate intracellular

39

faintly staining coccobacilli
small grayish colonies on GCBA

Francisella

40

needs agglutination test and dfat for confirming its spp

Francisella

41

BSL number/level for francisella

3

42

Zoonotic [2]



>Brucella
>Francisella

43

Bioterrorism agent [2]

>Brucella
>Francisella

44

Serological Tests for Spp ID
[2]

>Brucella
>Francisella

45

CA of tularemia
potential agent of bioterrorism

F. tularensis

46

zoonotic infection: rabbits

F. tularensis

47

intracellular bacteria which resists phagocytosis

F. tularensis

48

pathology:
skin ulcers at infection site
lymph node infection [ulceroglandular]
lungs [pneumonic]
GI system [typhoidal]

F. tularensis

49

severe pathology by f. tularensis

pneumonic infection

50

causes diarrhea similar to Salmonellosis

F. tularensis

51

Causes rabbit fever

F. tularensis

52

diagnosis for F. tularensis

DFAT
agglutination testing

53

grows on BCYE and Thayer-Martin w/ 10% CO2
Slow grower [3-4 weeks]

Brucella

54

zoonotic: infects cows and cattles

Brucella

55

specimen for DX of brucella

BM, Blood

56

spp of brucella closely related with abortion among cows and cattles

B. abortus

57

diagnosis of brucella

cultures

58

spp of id of brucella

serological test= phage and dye sensitivty

59

requires phage and dye sensitivty

brucella

60

causative agent of whooping cough

B. pertussis

61

faintly staining gram(-) coccobacilli

francisella
bordetella

62

mercury droplet colonies

b. pertussis

63

culture media for b. pertussis

bordet gengou agar
regan-lowe [charcoal horse blod agar]

64

secondary stain is replaced with stronger dye since this organism is faintly staining

b. pertussis

65

Example of stronger dye

carbolfuchsin

66

3 stages of pathology of b. pertussis whooping cough

catarrhal
paroxysmal
convalescent

67

3 stages of pathology of b. pertussis whooping cough
very mild inflammation
small mucus secretions

catarrhal

68

3 stages of pathology of b. pertussis whooping cough
intermittent fever
body malaise
fullblown infection

paroxysmal

69

3 stages of pathology of b. pertussis whooping cough
recovery stage

convalescent

70

causes mild respiratory infections in human
weaker strain of bordetella

b. parapertussis

71

causes cough in dogs [bordetella]

b. bronchiseptica

72

grows well in nonselective agars but not MAC

P. multocida

73

very suscep to penicillin easy to treat

P. multocida

74

CA of cellulitis w/c may progress to osteomyelitis, joint infection, pneumonia

P. multocida

75

thin poorly stained Gram - bacilli

Legionella

76

requires L-cysteine for growth

Legionella

77

legionella requires ___ for growth

L-cysteine

78

CM for Legionella

BCYE

79

Does Legionella grow on SBA

No

80

CAP appearance of Legionella

tiny colonies

81

is Legionella sac or nonsac

assacharolytic

82

most biochem tests are negative

Legionella

83

not easily identified by routine tests

Legionella

84

1sr discovered in 1976, American Legion in Philadelphphia

Legionella

85

what causes Legionellosis, Legionnaire's Disease, Pontiac Fever

L. pneumophila serogroup 1

86

specimen for ___
secretion from lower RT
cough swab
bronchial swab
expectorated sputum
cough plate

L. pneumophila serogroup 1

87

unique Dx test for Legionella pneumophila

urine antigen test

88

other Dx test for Legionella pneumophila [3]

urine antigen test
DFAT
Nucleic acid probes

89

very small Gram - variable coccobacilli

Garnerella vaginalis

90

how to differentiate G. vaginalis from Lactobacillus

Lactobacillus is large Gram + bacilli

91

2 similar spp recovered in vaginal area or secretions

Lactobacillus
G. vaginalis

92

associated with bacterial vaginosis, pelvic inflammatory disease [PID], postpartum sepsis

G. vaginalis

93

presence of __ is suggestive of BV due to G. vaginalis

clue cells

94

squamous epithelial cells with intracellular G. vaginalis

clue cells

95

2 possible disease if there is lower abdominal pain

UTI
Vaginalis

96

Dx for G. vaginalis

Amsel and Nugent scoring system

97

____ are too sensitive, not ideal for ideal for diagnosing Bacterial vaginosis by G. vaginalis

cultures

98

used for reading the pap smear [for possible infections by G. vaginalis]

Amsel and Nugent scoring system

99

____ do not meet criteria for BV by G. vaginalis

Cultures

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