BACTE LEC - Non-fermenting Gram (-) Bacilli to Pseudomonadaceae Flashcards Preview

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Flashcards in BACTE LEC - Non-fermenting Gram (-) Bacilli to Pseudomonadaceae Deck (35):
1

O2 requirements of Non-fermenting Gram (-) Bacilli

AEROBIC

2

Fail to acidify OF media overlaid with Mineral oil and TSI

Non-fermenting Gram (-) Bacilli

3

Where are Non-fermenting Gram (-) Bacilli found

in most environments: ubiquitous

4

can withstand treatment with disinfectants

Non-fermenting Gram (-) Bacilli

5

resistant to most antimicrobials

Non-fermenting Gram (-) Bacilli

6

O2 requirements of Pseudomonas

strict aerobes

7

motility of Pseudomonas

motile

8

oxidase rxn of Pseudomonas

+ except for P. luteolus and P. oryzihabitans

9

Catalase rxn of Pseudomonas

+

10

cm for most Pseudomonas

MAC

11

Most are oxidizers, some assacharolytic, what family

Pseudomonas

12

most frequently isolated spp of Pseudomonas

P. aeruginosa

13

common cause of nosocomial infections

P. aeruginosa

14

CA of bacteremia, skin, wound infections, UTIs, endocarditis

P. aeruginosa

15

CA of swimmer's ear

P. aeruginosa

16

CA of jacuzzi/ hot tub syndrome

P. aeruginosa

17

CA of severe pneumonia among CF patients

P. aeruginosa

18

Virulence Factors of P. aeruginosa

LPS
Unusual porin channels
Pili
Capsule [Alginate]
Elastase, Collagenase, Protease
Exotoxin A
Hemolysin
Pyocyanin pigment

19

Rx of choice for P. aeruginosa

Aminoglycosides
Piperacillin
Ticarcillin
Ceftazidine
Cefepime

20

pigments of P. aeruginosa [4]

pyocyanin
pyoverdin
pyorubin
pyomelanin

21

odor of P. aeruginosa

grape like or tortilla like

22

temperature for P. aeruginosa growth

able to grow at 42C

23

Describe appearance of P. aeruginosa on BAP

Ground glass appearance: large, rough, dull grayish colonies w/ feathery edge

24

hemolytic rxn of P. aeruginosa on BAP

beta

25

appearance of P. aeruginosa on clear agars

visible pigment

26

appearance of P. aeruginosa on EMB

Colorless [non-fermenting]

27

TSI Rxn of P. aeruginosa

K/K

28

Will P. aeruginosa grow in Cetrimide Agar

Yes

29

2 Other Pseudomonas species
>normal flora, opportunistic
>low virulence
>assoc with UTIs, post surgical abscesses, empyema, septic arthritis

P. fluorescens and P. putida

30

Appear as dry, wrinkled, with buff/ light brown color (tough and adherent)

Pseudomonas stutzeri

31

Appear as non-wrinkled, flat colonies with yellowish brown pigment

Pseudomonas mendocina

32

Other Pseudomonas spp
>soil denitrifiers: use ammonium as sole nitrogen source
>can grow in aerobic condition

Pseudomonas mendocina
Pseudomonas stutzeri

33

Other Pseudomonas spp
>Usually contaminants
>

Pseudomonas alcaligenes
Pseudomonas pseudoalcaligenes

34

recovered in Japanese rice paddies

P. oryzihabitans

35

Other Pseudomonas spp
>Oxidase (-)
>Produce yellow non-diffusable pigments
>Colonies: wrinkled or rough

P. luteola
P. oryzihabitans

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