Bacte Lec Quiz 2 - Bacterial Cytology Flashcards Preview

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Flashcards in Bacte Lec Quiz 2 - Bacterial Cytology Deck (62):
1

describe the chromosome in DNA

single, circular, appear as diffuse, nucleiod or chromatin body

2

sac-like structure w/in cell membrane

mesosome

3

– found free in cytoplasm attached to cytoplasmic membrane

2. Bacterial ribosome

4

function of granules

food storage

5

component of granules (2)

polysaccharide like glycogen
lipids such as poly-β- hydroxybutyrate or polyphosphate

6

– special name of granules of Corynebacterium

*Babes Ernst bodies/ granules

7

- special name of granules of Mycobacterium

*Much granules

8

cytoplasmic cell structures (4)

DNA, bacterial ribosome, granules, endospores

9

– resistant, highly refractive bodies within cell
-unstained areas or stained with specific spore stains
-function: resistant/protective structure

Endospores

10

component of spores:

calcium dipicolinic acid

11

cell structure not destroyed by boiling

endospores

12

cell structure that will germinate into another bacterium under favourable conditions

endospores

13

function of endospores

ensure bacterial survival in harsh conditions

14

causative agent of anthrax

Bacillus anthracis

15

– spore in spore strip for sterilization using oven

*Bacillus subtilis var niger

16

– spore spore in spore strip for sterilization using autoclave

*Bacillus stearothermophilus

17

components of cell membrane

phospholipids & proteins and NO STEROLS

18

function of cell membrane

regulates transport across membrane
acts as an osmotic barrier
enzyme regulation

19

functions of cell wall

maintains shape
prevents bursting of cell

20

components of cell wall

peptidoglycan
teichoic acids
lipids

21

type of teichoic acids that provide rigidity

lipoteichoic acids

22

components of Gram + cell wall

thick peptidoglycan
teichoic acids

23

components of Gram - cell wall

thin peptidoglycan
outer membrane

24

component of outer membrane

protein, lipid, LPS

25

Functions of Outer Membrane:

 Barrier for hydrophobic compounds
 Acts as sieve through pores
 Attachment sites

26

type of cell wall
contain waxy layer and fatty acid (Mycolic acid – responsible for hard staining)

Acid Fast Cell wall

27

species which lack cell wall but they have sterols
-Gram staining & acid fast stain not useful

Mycoplasma and ureaplasma

28

surface polymers (3)

capsule, slime layers, glycocalyx

29

type of surface polymer discrete organized covering

-act as virulence factors - prevents phagocytosis

Capsule

30

components of capsule (2 poly)

polysaccharide, polypeptides

31

function of capsule

prevents or evades phagocytosis

32

ex of encapsulated bacteria:

Klebsiella pneumoniae or Diplococcus pneumoniae

33

-encapsulated organisms best viewed using ??? stain

negative stain

34

-more diffused surface polymer

Slime layers

35

function of slime layer

-inhibit phagocytosis
-aid in adhesion to host tissue

36

surface polymer: tangled mass of thin polysaccharide

Glycocalyx

37

function of glycocalyx

-aid in adhesion to host

38

cell appendages

flagella
pili (fimbriae)

39

component of flagella

protein

40

flagella is only found in

bacilli, NOT IN COCCI

41

term: no flagella

atrichous

42

term: one flagella

monotrichous

43

term: flagella at one or both ends

amphitrichous

44

term: multiple tufts at one end

lophotrichous

45

term: occur on all sides of bacterium

peritrichous

46

flagella may contain ??? properties

antigenic

47

antigens mentioned (4)

V, K, H, O

48

antigen from cell wall

O antigen

49

somatic antigen

O antigen

50

Ag from flagella

H antigen

51

Ag from capsule

K antigen

52

cell appendages are made of

protein

53

cell appendage: hairlike protein structures,

Pili (fimbriae)

54

pili function

aid in attachment to surfaces

55

2 types of pili

sex pili
adherence or ordinary pili

56

function of sex pili

reproduction: bacterial conjugation and gene exchange, for reproduction

57

function of adherence or ordinary pili

allows bacteria to attach to specific eukaryotic host cells (for attachment)

58

organism with sex pili

Neisseria gonorrhoeae

59

stain used for Cryptococcus neoformans

Negative stain

60

stain that stains background, not bacteria

Negative stain

61

stains used in negative stain

nigrosin dye
india ink

62

dye usually used for CSF to detect Cryptococcus neoformans

India ink

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