Bacte Lec - Staph Flashcards Preview

► Med Misc 05 > Bacte Lec - Staph > Flashcards

Flashcards in Bacte Lec - Staph Deck (96):
1

gram reaction of staph

positive

2

color of staph in gram stain

positive

3

gram + spherical cells which normally colonize the skin

micrococcus

4

morphology of micrococcus

cubes of 8

5

coagulase reaction of micrococcus

negative

6

coccus bacteria in marine environment
rarely assoc with human infection

planococcus

7

test to diff strep and staph

catalase test

8

catalase rxn of stapg

+

9

all staph are [aerobe or anaerobe]

facultative anaerobe

10

only obligate anaerobe staph

S. saprophyticus

11

hemolytic rxn of staph

beta or non-hemolytic

12

reaction of staph in catalase [describe]

bubble formation

13

catalase test not used on blood agar bec

blood has coagulase

14

rxn of staph on SIM

confined; not turbid

15

common isolate on wound infection

suppurative type of infection [pus-forming]

16

habitant of skin and mucous membrane

staph

17

found in environment on dust
inanimate objects

staph

18

single most impt test to differentiate pathogenic staph from other species

coagulase test

19

coagulase producing staph

Hyicus
Aureus
Delphini
Intermedius
Schleiferi

20

all coagulase producing staph are animal species except

aureus

21

virulence factors of staph [5]

enterotoxin
exfoliative toxin
cytolytic toxin
enzymes
protein A

22

type of VF
heat stable

enterotoxin

23

type of enterotoxin that are resistant to gastric acid and digestive juices

enterotoxin A and enterotoxin D

24

produce acid in the form of HCl

parietal cells

25

type of VF
phage group II
involvement of skin
formation of blisters

Exfoliative toxin

26

type of exfoliative toxin Observed among tampon

Toxic shock syndrome toxin 1

27

type of exfoliative toxin
sloughing off of epidermal layer of skin
scalded skin syndrome [Ritter disease]

epidermolytic

28

staph infection most observed where

skin

29

type of cytolytic toxin
platelets destroyed

alpha toxin/ hemolysin

30

type of cytolytic toxin
injury on most cells on culture and to leukocytes
less lethal

delta hemolysin

31

another name for leukocidin

Panton-Valentine

32

type of cytolytic
lethal to PMN [macrophage]
invasiveness of organism

Leukocidin

33

another name for beta hemolysin

hot cold lysin

34

attacks sphingomyelin of RBC membrane

beta hemolysin

35

enzymes as VF [3]

Coagulase
Hyaluronidase
Lipase

36

type of enzyme
clumping factor
virulence marker

coagulase

37

type of enzyme
coagulase + and coagulase -
acts on fats and oils
responsible for colonization

lipases

38

type of enzyme
hydrolyze hyaluronic acid
with exfoliation
tries to make skin loose so bacteria can spread

hyaluronidase

39

another name for hyaluronidase

Durans-Reynal Factor or Spreading factor

40

type of VF with phagocytic activity

protein A

41

epidemiology of staph [3]

anterior nares of human carriers
hospital outbreaks [common nosocomial infections]
infant nurseries, burn units, invasive procedures

42

transmission of staph [2]

direct contact
community-acquired infections
poor hygiene
fomite transmission [nonliving]
carriers

43

how to prevent s.aureus infection

hand washing

44

Cutaneous infection [4]

bullous impetigo
boils
furuncles
carbuncles

45

toxin induced diseases [4]

food poisoning [refrigerated foods rich with creamy sauces & mayonnaise]
toxic shock syndrome
scalded skin syndrome
toxic epidermal necrolysis

46

meaning of SSS

Scalded Skin Syndrome

47

meaning of TEN

toxic epidermal necrolysis

48

resolution for toxic induced diseases

steroid therapy

49

disease observed among young menstruating females

toxic shock syndrome

50

common among sexually active young women

S. saprophyticus

51

also seen in older men with indwelling catheter

S. saprophyticus

52

resistant to novobiocin

S. saprophyticus

53

most common hospital acquired UTI

catheter infection

54

causes UTI

S. saprophyticus

55

associated with pyelonephritis and cystitis

S. saprophyticus

56

a multisystemic disease characterized by high fever, hypotension, shock
other conditions are pneumonia, endocarditis, osteomyelitis, septic arthritis

toxic shock syndrome

57

isolation and identification culture media used [4]

thioglycollate broth
MSA
PEA
CNA

58

CNA meaning

columbia colistin nalidixic agar

59

PEA meaning

phenylethyl alcohol agar

60

MSA meaning

mannitol salt agar

61

cm which differentiates S. aureus from other staph

MSA

62

cm which eliminates Gram (-) only gram + is allowed to grow

PEA

63

colony size of aureus, epidermidis, saprophyticus

pinhead

64

type of staph beta hemolytic

aureus

65

type of staph non-hemolytic [2]

epidermidis and saprophyticus

66

color of colonies of aureus

yellow, tan, orange

67

color of colonies of epidermidis

white

68

color of colonies of saprophyticus

yellow

69

a clear zone indicates

hemolytic rxn

70

substrate of catalase test

3% hydrogen peroxide

71

genus + for catalase

staph

72

genus - for catalase

strep
enterococcus

73

blood used in coagulase test

citrated blood

74

coagulase + bacteria

s. aureus

75

coagulase - bacteria

epidermidis or saprophyticus

76

novobiocin susceptible

epidermidis

77

novobiocin resistant

saprophyticus

78

coagulase test - for cell bound coagulase

slide test

79

rgts in slide test

human, rabbit, dog plasma

80

type of glass slide used in slide test

smoked glass slide

81

coagulase test for free coagulase

tube coagulase test

82

number of hours of incubation for tube coag test

4 hrs

83

why is there a need to check every time for the clot during the whole incubation period

S. aureus has hyaluronidase which can liquefy the clot = false negative

84

in msa, what does a yellow color mean

acid fermentation/LF

85

in msa, what does a red color mean

alkaline

86

what type of test rxn involves clearing of toluidine blue

DNAse Test Reaction

87

base medium of DNAse Test Reaction

nutrient agar

88

reagent in DNAse Test Reaction

dilute HCl

89

in DNAse Test Reaction, it is an indication of nucleic acid hydrolysis

blue green to pink

90

no clearing means what in DNAse Test Reaction

DNA not utilized

91

(+) in DNAse Test Reaction means

Hydrolysis of DNA

92

agar in gelatinase test

gelatin

93

reaction of gelatinase producing organism in gelatinase test

gelatin will liquefy

94

test to differentiate saprophyticus from other species

thermostable nuclease test/ novobiocin testing

95

s. aureus enzyme stable at what temp

40C

96

Other methods: rapid testing

SEROSTAT
Slide Test
Staphylatex
Accu-staph
staphurex
staphyloslide
hemastaph
staphase

Decks in ► Med Misc 05 Class (295):