Basic lab/aseptic technique

Question 1

Wear appropriate gear

Answer 1

Gloves, lab coat, closed toed shoes, tied-back hair (if long), goggles, no loose jewelry or clothing items.

Question 2

Sanitize your workplace and any utensils.

Answer 2

Wipe down your lab bench with a disinfectant (spirling motion), and sanitize your gloves in order to reduce any chance of cross contamination from your lab environment into the samples.
Sterilize appropriate reusable equipment over a bunsen burner

Question 3

Do not reuse micropipetting tips for different samples. Dispose of them appropriately. (T/F)

Answer 3

True

Question 4

Always recalibrate your micropipette back to its ________ after use.

Answer 4

maximum

Question 5

Avoid exposing surface-sensitive equipment to the environment for more than necessary

Answer 5

Do not leave micropipet tips open
Do not leave your sample tubes open
Use clamp-shell technique while working with agar dish plates