Basics Of Multiphoton, Superresolution And Structures Illumination Microscopy Flashcards Preview

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Flashcards in Basics Of Multiphoton, Superresolution And Structures Illumination Microscopy Deck (6):
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Examples of super-resolution methods

Structured illumination
- SR-SIM

Spatially patterned excitation
- STED
- RESOLFT

Localization methods
- STORM
- PALM

1

SR-SIM

Structured illumination super resolution microscopy

2

STED

Stimulated emission depletion
- drive down to ground state with second dump pulse before molecule can fluoresce

3

STORM

Stochastic optical reconstruction microscopy

4

PALM

Photo activated localization microscopy

- image only some dye molecules at one time

5

De convolution microscopy

Operates on principle of PSF
As one moves away from focal plane in which the object lies, the light from it will spread in a predictable manner