Biochem Day 1 Flashcards Preview

NBDE Anatomic Sciences > Biochem Day 1 > Flashcards

Flashcards in Biochem Day 1 Deck (18):
1

Alanine aminotransferase in serum diagnosis 

Viral Hepatitis

2

Aspartate aminotransferase diagnosis in serum

Alcoholic hepatitis

Myocardial infarction

3

Alkaline Phosphatase serum marker diagnosis

Osteoblastic bone diseases (Pagets, prostate cancer)

Obstructive liver disease

4

Amylase serum diagnosis

Mumps

Acute pancreatitis

5

Creatine kinase serum diagnosis

Myocardial infarction

6

Lactate DH serum diagnosis

Myocardial infarction

7

What rxn does carbonic anhydrase catalyze

CO2 + H2O= HCO3- + H+

Fastest known enzyme

Also speeds up formation of carbonic acid

8

Where is carbonic acid found

RBC

Kidney (Reabsorption of bicarbonate at proximal tubule)

9

How are zymogens activated

Removal of peptide fragment

10

How are pancreatic zymogens release and activation mediated

Mediated by cholecystokinin, secretin, parasympathetic NS

11

Stomach ztmogens

Pepsinogen

12

Pancreas zymogens 5

  • Chymotrypsinogen
  • Trypsinogen
  • Procarboxypeptidase A
  • Procarboxypeptidase B
  • Preelastase

13

Von Gierkes disease deficiency in

Deficient in Glucose-6-phosphatase

Needed to convert glucose-6-phosphate==> Glucose

14

Gluconeogenesis function and site

Glucose is made from carbohydrates (primarily amino acids, NOT FA)

Occurs in liver 

Provides glucose to other tissues when glucose is low 

Often lactic acid or AA are converted to pyruvate or phosphoenolpyruvate to be made into glucose

15

Energy for Gluconeogenesis comes from 

Fatty acid oxidation

16

What type of enzyme inhibition can be overcome

Competitive inhibition

Noncompetitive cannot be overcome( includes allosteric inhibitors) can bind free enzymes and ES complexes

17

What does a uncompetitive inhibitor bind to

Binds to an enzyme that already has a substrate bound 

18

Aspirin is an example of what type of inhbitor

Irreversible inhibitor

Destroy functional group

Aspirin (acetylsalicylate) irreversibly inhibits cyclooxygenase by acetylating the active site serine