Biochem Lab Color Rxns Flashcards Preview

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Flashcards in Biochem Lab Color Rxns Deck (30):
1

purpose of biuret test

detect presence of peptide bonds

2

purpose of ninhydrin

presence of alpha AA

3

purpose of xanthoproteic

presence of aromatic AA

4

purpose of millon's

presence of tyrosine (phenolic group)

5

purpose of hopkins-cole

presence of indole group of tryptophan

6

purpose of sakaguchi

un or monosubstituted guanidine in arginine

7

purpose of nitroprusside

presence of cysteine

8

purpose of fohl's

presence of sulfur-containing aa

9

purpose of test for amides

R groups of glutamine and arginine

10

purpose of pauly

test for tyrosine and histidine

11

(+) result of biuret

violet solution

12

(+) result of ninhydrin

blue-violet sol'n

13

(+) result of xanthoproteic

yellow ppt/soln

14

(+) result of millon's

red/reddish-brown solution

15

(+) result of hopkins-cole

purple ring at junction where 2 liquids meet

16

(+) result of sakaguchi

red coloration

17

(+) result of nitroprusside

red soln

18

(+) result of fohl's

black or brown sediment

19

(+) result of test for amides

red litmus to blue
evolution of gas

20

(+) result of pauly test

red coloration

21

reagents used in biuret

2.5 M NaOH
0.1 M CuSO4

22

reagents used in ninhydrin

0.1% ninhydrin solution

23

reagents used in xanthoproteic

Conc. HNO3
Conc. NaOH

24

reagents used in millon's

Millon's reagent

25

reagents used in hopkins-cole

Hopkins Cole reagent
H2SO4

26

reagents used in sakaguchi

10% NaOH
0.02% Naphthol solution
2% NaOBr

27

reagents used in nitroprusside

3M NaOH
2% Nitroprusside

28

reagents used in fohl's

30% NaOH
5% (CH3COO)2Pb

29

reagents used in test for amides

20% NaOH

30

reagents used in pauly

Diazo reagent (1% sulfanilic acid + 5% NaNO2 solution)
10% Na2CO3

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