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Flashcards in Biochemistry Deck (17):
1

What is the equation for Vmax?

Vmax=[E]kcat

2

What is the definition of kcat?

substrate turnover/[E]/s

3

What is the definition of km?

[S] when 50% of the enzyme's active sites are saturated and indicates enzyme's affinity for substrate

4

What is the equation for catalytic efficiency?

kcat/km --> high kcat or low km indicates high catalytic efficiency

5

What is the equation for enzyme v?

v= (Vmax [S]) / (km + [S])
= ([E] [S] kcat)/ (km + [S])

6

What is the glycosidic bond in sucrose?

glucose-a-1,2-fructose

7

What is the glycosidic bond in lactose?

galactose-b-1,4-glucose

8

What is the glycosidic bond in maltose?

glucose-a-1,4,-glucose

9

What is the glycosidic bond in cellulose?

b-1,4 (unbranched)

10

What is the glycosidic bond in starch?

amylose (unbranched): a-1,4
amylopectin (branched): same + a-1,6 linked branches

11

What is the glycosidic bond in glycogen?

a-1,4 (unbranched) and a-1,6 (branches)

12

anhydrase

take away water

13

hydrase

adds water

14

carbonic anhydrase

Got its name because it removes water from H2CO3 (carbonic acid) BUT catalyzes the reaction in both directions

15

Enzymes

Only speed up the reaction they do not change the equilibrium.

16

Beta-oxidation of even numbered FA

n= #C/2= #acetyl CoAs
n -1= number of rounds of B-ox because last one generates 2 acetyl CoA

17

Beta=oxidation of odd numbered FA

n= #C/2= #acetyl CoAs
n -1= number of rounds of B-ox because last one generates 1 acetyl CoA (2C) and 1 Succinyl CoA (3C)
Succinyl CoAs is only produced in the final step for odd-numbered FA chains