BIS 103: The Electron Transport Chain Overview

Question 1

What takes place in the Matrix?

Answer 1

PDH complex
Krebs
B oxidation
amino acid ox

Question 2

What proteins are in the inner membrane?

Answer 2

complexes 1-IV : respiratory e- carriers
ADP/ATP translocase
ATP synthase

Question 3

what are mito infoldings called?

Answer 3

cristae

Question 4

What is the MW limit of mito pores?

Answer 4

5000 MW

Question 5

what portion of protein are made from mito DNA?

Answer 5

20%

Question 6

What enables mixin contents in mitochondria?

Answer 6

fusion and fission

Question 7

Rieske Iron-Sulfur center

Answer 7

2Fe-2Se, with 1 Fe bound to 2 cys, other Fe bound to 2 his

Question 8

Draw the different iron sulfur complexes

Answer 8

: ]

Question 9

Redox potential of Fe-S complexes

Answer 9

not absolute - dependent on protein to which is bound

Question 10

Ubiquinone ring structure

Answer 10

10 isoprene units - very hphobic to stay in membrane

aromatic ring - stabilizes structure, reactive o’s

Question 11

Ubiquinone rxns - names

Answer 11

add e- and h+

• > semiquinone radical - 1O*, 1 OH
• > ubiqinon - 2 oh’s

Question 12

goal of ubiqinone

Answer 12

holds h+ and e-, in 1 or 2 pairs at a time

Question 13

importance of heme rings to scientists

Answer 13

indicates e- passage by changing spectra - something the backbones and R groups can’t do

Question 14

Heme A differs from others because:

Answer 14

has hphobic tail to embed in membranes

Question 15

Route of FADH2 e-

Answer 15

complex 2 -> Q ->complex 3 -> cytochrome c -> complex 4

Question 16

Route of NADH e-

Answer 16

compelx 1: Q ->complex 3 -> cytochrome c -> complex 4

Question 17

route of H+ per NADH - amounts, totals

Answer 17

complex I - 4
complex 3 - 4
complex 4 - 2

10 total

Question 18

route of H+ per FADH2 - amounts, totals

Answer 18

complex 3 - 4
complex 4 - 2

6 total

Question 19

mobile carriers

Answer 19

coQ, cytochrome c

Question 20

Where does cytochrome C work?

Answer 20

intermembrane space

Question 21

which side of the mitochondria has a + chaarge?

Answer 21

intermembrane space

Question 22

where is the N side?

Answer 22

the matrix

Question 23

average resp e- carriers, differences

Answer 23

10^4 - hearts have more

Question 24

Complex 2 other names

Answer 24

succinate coQ oxidoreductase

succinate dehydrogenase

Question 25

Whis complex doesn’t transport protons? Why?

Answer 25

Complex II - doesnt harvest enough energy from FADH’s e-

Question 26

Which complex has 2 enzymatic sites?

Answer 26

Complex 2

Question 27

Which complex is made of 2 dimers? what are they for?

Answer 27

complex 2

caves for coQ to bounce around

Question 28

Which complex is th elargest?

Answer 28

complex I

Question 29

Which complex has 2 binuclear sites? What is a binuclear site? What are they?

Answer 29

Complex 4
2 metals close enough to act as 1
2 Cu, Cu-Fe to make water