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Flashcards in blood - phyana lab Deck (87):
1

functions of blood

transportation
regulation
protection

2

composition of blood

55% plasma
45%formed elements

3

composition of plasma

91.5% water
7% proteins
1.5% other nutrients

4

proteins in plasma

albumin
globulin
fibrinogen

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(54%): function in osmosis; carriers

albumin

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(38%): serve as antibodies

globulin

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(7%): important in clotting

fibrinogen

8

types of agranulocytes

nk cells
lymphocytes
monocytes

9

Carries 98.5% of O2 and 23% of CO2

hemoglobin

10

RBC count (gen, males, females)

RBC count: about 5 million/µl
Male: 5.4 million cells/µl; female: 4.8 million/µl

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structure of RBC which provides for maximal gas exchange
Is flexible for passing through capillaries

Lack of nucleus causes biconcave disc shape with extensive plasma membrane

12

Appear white because of lack of hemoglobin

WBC

13

Normal WBC count:

5,000-10,000/µl

14

WBC count usually increases in

INFECTION

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Two major classes based on presence or absence of granules in WBC

Granular: neutrophils, eosinophils, basophils

Agranular: lymphocytes, monocytes

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usually make up 2/3 of all WBCs

Neutrophils

17

Maj function of WBC

defense against
Infection and inflammation
Antigen-antibody (allergic) reactions

18

wbc Life span:

typically a few hours to days

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high WBC count in response to infection, exercise, surgery

Leukocytosis

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low WBC count

Leukopenia

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from megakaryocytes

platelets

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normal count of platelets

150,000-400,000/µl blood

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functions of platelets

Plug damaged blood vessels
Promote blood clotting

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life span of platelets

Life span 5–9 days

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Life span of RBC

3-4 months or 120 days

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Blood types in each person are determined by

genetics

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antigen of Type A

A antigen

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antigen of Type B

B antigen

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antigen of Type AB

A antigen and B antigen

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antigen of Type O

No antigens

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antibody for Type A

anti-B

32

antibody for Type B

anti-A

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antibody for Type AB

none

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antibody for Type O

anti-A and Anti-B

35

RBCs with Rh antigen

Rh+

36

RBCs without Rh antigen

Rh-

37

agglutination with anti-D

Rh+

38

NO agglutination with anti-D

Rh-

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color of anti-B

yellow

40

color of anti-A

blue

41

color of anti-D

colorless

42

If mismatched blood (“wrong blood type”) given

antibodies bind to antigens on RBC and RBC hemolyse

43

Type AB called “universal recipients” because have no

anti-A or anti-B antibodies

44

Type O called “universal donors” because have neither

A nor B antigen on RBCs

45

why is type O being a universal donor misleading?

Misleading because of many other blood groups that must be matched

46

unclotted blood

plasma

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part of the blood with anticoagulants

plasma

48

clotted blood

serum

49

part of the blood without anticoagulants

serum

50

expired rbc indication

reddish plasma because of hemolysis

51

wbc for parastic and allergic rxn

eosinophil

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wbc for viral infection

lymphocytes

53

wbc for bacterial infection

neutrophil

54

low RBC

anemia

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what temp must platelets be stored to avoid disintegration

room temp

56

blood type is affected by (2)

genetics
pregnancy

57

2 methods of blood typing

forward and reverse

58

method of blood typing which makes use of anti-sera

forward

59

methods of blood typing which makes use of known serum

reverse

60

Another term for antigens

Agglutinogens

61

Origin of blood since it is a CT

Mesenchyma

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Portion of blood that has no fibrinogen

Serum

63

Liquid portion of clotted blood

Serum

64

The formed elements of blood are suspended in the -----

Plasma

65

Any surface that comes into contact with blood must be ----

Disinfected

66

One of the simplest most accurate tests in hematologic investi

Hematocrit

67

Thsi readinf gives the percentage of erythrocytes in whole blood

Hematocrit

68

What capiklary tube must be used for blood samples with anticoagulant

Non heparinized blue

69

Sealed end of capillary tube must br positioned at the ------

Periphery

70

The darker the color of the sample

The higher the hemoglobin content

71

Components of hemacytometer

Counting chamber
WBC pipet
RBC pipet

72

Accessory devices of hemacytometer

Thick cover slips
Suction device

73

An ordinary cover slip cannot be used in hemacytometry because

It has uneven surface

74

Characteristics of good WBC

Hypotonic
Easily prepared
Cheap
Readily available
Good preservative

75

If the diluting fluid gors beyond 11 mark or if there re bubbles,

Discard mixture and start fron the beginning

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angle of the pipette while charging

30-35

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In WBC or RBC count secure the pipette using the ------ and ----- finger

Thumb and middle finger

78

Characterisitcs of good RBC diluting fluid

Easy to prepare
Cheap
Good preservative
High specific gravity
Isotonic
Has a buffer action
Does not initiate the growth of molds

79

Another term for antibodies in the plasma

Agglutinins

80

If a certain agglutinogen is present, its corresponding agglutinin must be _____

Absent

81

Materials used in blood typing

Blood Lancet must be sterile
Applicator stick
Antiserum A B D
70% alcohol

82

Time that elapses from the appearance of the first drop of blood to the time the bleeding stops representa

bleeding time

83

Materials used in bleeding time

Blood lancet
Filter paper

84

---- surface can shorten bleeding time

Rough

85

What was done to demonstrate active hyperemia

Left index finger was immersed in hot water 60C

86

What was done to demonstrate passive hyperemia

Wind a rubber band tightly above the second interphalangeal joint of thr left index finger

87

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