Flashcards in C2.1.6 - Chromatography Deck (20):
How many chemical phases are in chromatography?
What are the chemical phases in chromatography called?
Stationary and mobile
What is the difference between a stationary and mobile phase?
Stationary - cannot move
Mobile - can move
What is the stationary phase in paper chromatography?
What is the mobile phase in paper chromatography?
What is the stationary phase of thin-layer chromatography?
A thin layer of silica or alumina powder spread over a plate of glass or plastic
What is paper and thin-layer chromatography used for?
Separating soluble coloured substances
Explain the process of thin-layer chromatography
Put the solvent into a chromatography tank to a depth of about 1cm. Add a small amount of sample, taking care not to damage the powder on the plate. Let the solvent travel through the powder, and take the plate out before it reaches the top. Analyse the pattern of coloured spots
What is a chromatogram?
The graph that is created to analyse the results
What is the chromatography tank used for?
To place the substances in to start chromatography
What does the pattern produced in chromatography depend on?
How each component was distributed during each of the chemical phase
What would happen if the component forms a stronger bond with the mobile phase than the stationary phase?
It moves further up the plate
What happens if the component creates a stronger bond with the stationary phase than the mobile phase?
The component doesn’t move very far up the plate
What is the stationary phase in gas chromatography?
Silica or alumina powder packed into a metal column
What is the mobile phase in gas chromatography?
An unreactive carrier gas
What does gas chromatography do?
It separates the components of a mixture, as well as measure their amounts
Name all the equipment used in gas chromatography
Carrier gas cylinder
What does the carrier gas cylinder do?
It supplies the mobile phase
Where is the metal column located in during gas chromatography?