Carbohydrate Metabolism Glucose Regulation and Formation Flashcards Preview

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Flashcards in Carbohydrate Metabolism Glucose Regulation and Formation Deck (30):
1

Anabolism

energy-requiring synthesis of more complex products

2

Catabolism

energy-yielding degradation of complex precursors

3

Pancrease

cell: islet cells
metabolic function: releases insulin, glucagon, and numerous hormones

4

Liver

Cell: hepatocyte
Metabolic function: maintains metabolic homeostasis by normalizing blood glucose and synthesizing and degrading glycogen, glucose, FAs and NAs, proteins, and ketone bodies

5

Muscle

Cell: skeletal, smooth, and cardiac muscles
Metabolic function: utilizes glucose, FAs and AAs to fuel muscular activity

6

Adipose Tissue

Cell: adipocyte
Metabolic function: stores energy via lipids very static

7

Key regulators

insulin
glucagon
epinephrine (adrena medulla)
glucose
ATP/AMP

8

Major fates of glucose, four of them ***

source of ATP (pyruvate)
energy storage (glycogen)
molecular precursor (ECM and cell wall polysacchs.
structural backbone (ribose 5-phosphate)

9

Major pathways of Carbohydrate metabolism

1. respiration --> forms ATP
2. Storage --> forms glycogen, glucose
3. Regenerative--> forms glucose (in liver)
4. Synthetic--> forms NAs, lipids and AAs

10

Some metabolic pathways are exclusive which ones are they?

glycolysis and gluconeogenesis (liver)
glycogenesis and glycogenolysis
**if one is occurring then the other cannot happen at the same time**

11

Aerobic catabolic pathways

Citric acid cycle, and ETC(oxidative phosphorylation)
also glycolysis

12

anaerobic pathways

glycolysis

13

Glycogen stores intracellular glucose
structures and where they are found

branched glucose homopolysaccharide
primary mechanism for intracellular energy storage
primarily found in the liver(10% dry weight) and muscle(2%)
necessary to maintain cellular osmolarity
forms large molecular complexes stored in granule organelles

14

What starts the formation of glycogen and how many glucose molecules per chain in glycogen?

glycogenin
12 to 14 glucose residues

15

Glycogen pathway

1.glucose-> glucose 6-phosphate
2.glucose 6-phosphate -> glucose 1- phosphate
3.glucose 1-phosphate -> uracil diphosphate glucose
4.uracil diphosphate glucose -> glycogen chain
5.glycogen chain -> glycogen particle
enzymes
1. hexokinase
2. phophoglucomutase
3. UDP-glucose pryphosphorylase
4. glycogen synthase (point of regulation)
5. glycogen branching enzyme

16

Glycogenolysis

-Glycogen is broken down via glycogen phosphorylase and releases glucose-1-phosphate molecules
-once about 4 residues are left a transferase activity of debranching enzyme takes 3 of the four glucose molecules and puts them on the end of the chain.
- the one remaining glucose is cut via (alpha 1-6 glucosidase activity of debranching enzyme) and a free glucose is released.

17

What are the two fates of the glucose 1-phosphate molecules from the glycogen?

1. phosphoglucomutase converts it to glucose 6-phosphate and off the glycolysis
2. ONLY in LIVER!! the Glucose 6-phosphate can enter the liver and go to the ER of the liver where the enzyme **Glucose 6-phosphatase** removes the phosphate and releases the free glucose to the blood.

18

Glycogen regulation at glycogen synthase

-regulated by GSK3
-When glycogen synthase is phosphorylated it is inactive and is in the glycogen synthase b form
-When insulin is present is inactivates GSK3 and activates PP1 where glycogen synthase is activated.
--it is also activated by glucose-6-phosphate and glucose
inactivated by glucagon and epinephrine

19

Glycogen phoshorylase regulation

phosphorylase b is inactivated and is not phosphorylated but glucagon and epinephrine activate phosphorylase and lead to it being phosphorylated and activated.

kind of the opposite of glycogen synthase regulation with being phosphorylated or not

20

insulin signaling

activates glycogenesis via
IRS-1 which activates PI3K to activate PIP2 to activate PIP3 which in turn activates PKB which activates GSK3 to activate glycogen synthase
**insulin activates GLUTs, hexokinase, and glycogen synthase**

21

Epinephrine and glucagon in the liver

increase glycogenolysis and gluconeogensis to increase blood glucose
glycolysis is decativated
**goal is to create more glucose 6-phosphate for the blood

22

epinephrine in the muscles

increases glycogenolysis and glycolysis so that the liver can get more glucose to the blood

23

phosphorylase allosteric regulators

glucose (slows glycogenolysis)
insulin ( increases glycogenolysis)

24

gluconeogensis where is occurs and the process

occurs in all cells but mostly in the liver
lactate or pyruvate is converted to glucose
**energetically costly 4 ATP 2 GTP 2 NADH + H

25

what is the cori cycle

Glycogen is broken down into lactate in muscle
lactate is released to blood and goes to liver
in the liver lactate is made into glucose via gluconeogensis (uses ATP). The glucose is then released into blood.

26

What is the point of regulation for gluconeogenesis

the fructose 1,6-bisphosphatase -1 (FBPase-1) enzyme which converts fructose 1,6-bisphosphate to fructose 6-phosphate

27

What energy substrates regulate the FBPase-1 and PFK-1 (glycolysis)

AMP inhibits it FBP-1 (poor energy substrates)
AMP and ADP activate PFK-1 (poor energy substrates)
citrate and ATP inactivate PFK-1 (energy rich substrates present)

28

AMP kinase (AMPK) detects intracellular AMP
how?

regulating different organ systems by measuring the amount AMP present. This will change metabolic states in the body.
inhibits pancreas from releasing insulin, liver from FA synthesis and cholesterol synthesis, adipose tissue from FA synthesis and lipolysis.
basically tells the body hey we need energy get going.

29

State of feeding, what happens in the pancreas, liver, adipose, and muscle?

pancreas: insulin release
liver: glycogen and FA production
adipose: lipid storage, glycolysis, and lipid production
muscle: glycolysis and glycogen production

30

State of fasting, what happens in the pancreas, liver, adipose, and muscle?

pancreas: glucagon
liver: gluconeogenesis, glycogenolysis, ketone and glucose release
adipose: FA and glycerol release
Muscle: ketone metabolism, reduced glycolysis