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Flashcards in Cbc expt Deck (37):
1

Universal precaution

Treat all body fluids infectious

2

Tests in CBC

Hemoglobin measurementHematocrit measurementRBC countWBC countDifferential Count

3

MEASURES Volume of packed red cell after centrifugation

HEMATOCRIT measurement

4

METHOD IN hematocrit measurement

Adam’s micromethod

5

materials in hematocrit measurement

capillary tube (red, blue) sealing clay/paraffin wax microhematocrit centrifuge machine microhematocrit reader

6

Procedure in hematocrit measurement

Fill capillary tube with blood (3/4)Seal. LabelCentrifugate. Use balancerSpin 10,000 rpm 4-5min

7

in hematocrit measurement, spin at what rpm and how many min

10,000 rpm 4-5min

8

Hematocrit NT

male- 47 +/- 7 vol% / 0.47 +/- 7 female- 42 +/- 5 vol%

9

Methods for hemoglobin measurement

acid-hematin method Cyanmethemoglobin method

10

Method for hemoglobin measurement that gives less error

Cyanmethemoglobin method

11

MATERIALS FOR hemoglobin measurement

0.01 N HCl Sahli’s tube (w/comparator block) Sahli’s pipette dH20 Stirring rod

12

PROCEDURE FOR hemoglobin measurement

Deliver 0.01N HCl into Sahli’s tube – 2markAspirate 0.02 mL bloodExpel blood into Sahli’s tubeRinse pipette with dH20-add into the tubeAdd dH20 drop by drop – comparator blockRead lower miniscus

13

NR hemoglobin measurement

NR: male : 14-16.5 g/100mL or 14-16.5g% 140-165 g/L female: 12-15g/100mL

14

Used in counting RBC, WBC, Platelet, absolute eosinophil count, sperm countWith thick cover slipImproved Neubauer Counting Chamber

hemacytometer

15

specific hemacytometer in the lab

Improved Neubauer Counting Chamber

16

# of WBC in 1 cu mm of bloodDil fluid must lyze RBC

WBC count

17

materials for WBC count

WBC pipetteDil fluidhemacytometer

18

procedure for wbc count

Aspirate blood 0.5 mark. Wipe sidesAspirate RBC dil fluid up to 101 mark. Mix dil’n 1:200Discard 5-6 gttCharge counting chamberStand 5minCount RBC in HPO

19

materials for differential count

Blood2 slidesDifferential stainPasteur pipetteCedar wood oil

20

High refractive index, thereby increasing the numerical aperture of the objective lensHave specific optical and viscosity characteristics

cedar wood oil

21

describe cover glass used in differential count

thicker, less flexible, surface tension of the fluid will not deform them, height of the fluid is standardized

22

procedure for differential count

Prepare smearDry smear on flat surfaceStain smearCount 100 cells in OIO

23

technique used in differential count

strip differential technique

24

how many WBC must be counted in a smear

100

25

devices used for DIFFERENTIAL COUNT

hand tally counterdifferential counter

26

how many quadrants are there for WBC

4

27

What are counted in WBC or RBC

dots

28

how many secondary squares are in improved neubauer

9

29

how many tertiary squares are in 1 WBC square

16

30

what is the total number of tertiary squares of a WBC

16 x 4 = 64

31

1 RBC square = ___ tertiary squares

5

32

1 RBC tertiary square  =___ small squares

16

33

total number of small RBCsquares 

16 x 5 quadrants = 80 small squares

34

what are the staining solutions in differential count (list in order)

1st: ethanol2nd: eosin3rd: methylene blue4th: buffer or distilled water

35

staining solution for fixing the slide

ethanol

36

staining solution for staining

eosinmethylene blue

37

staining solution for rinisng solution or stains

buffer or distilled water

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