CBC Expt - phyana lab Flashcards Preview

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Flashcards in CBC Expt - phyana lab Deck (38):
1

Universal precaution

Treat all body fluids infectious

2

Tests in CBC

Hemoglobin measurement Hematocrit measurement RBC count WBC count Differential Count

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MEASURES Volume of packed red cell after centrifugation

HEMATOCRIT measurement

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METHOD IN hematocrit measurement

Adam’s micromethod

5

materials in hematocrit measurement

capillary tube (red, blue) sealing clay/paraffin wax microhematocrit centrifuge machine microhematocrit reader

6

Procedure in hematocrit measurement

Fill capillary tube with blood (3/4) Seal. Label Centrifugate. Use balancer Spin 10,000 rpm 4-5min

7

in hematocrit measurement, spin at what rpm and how many min

10,000 rpm 4-5min

8

Hematocrit NT

male- 47 +/- 7 vol% / 0.47 +/- 7 female- 42 +/- 5 vol%

9

Methods for hemoglobin measurement

acid-hematin method Cyanmethemoglobin method

10

Method for hemoglobin measurement that gives less error

Cyanmethemoglobin method

11

MATERIALS FOR hemoglobin measurement

0.01 N HCl Sahli’s tube (w/comparator block) Sahli’s pipette dH20 Stirring rod

12

PROCEDURE FOR hemoglobin measurement

Deliver 0.01N HCl into Sahli’s tube – 2mark Aspirate 0.02 mL blood Expel blood into Sahli’s tube Rinse pipette with dH20-add into the tube Add dH20 drop by drop – comparator block Read lower miniscus

13

NR hemoglobin measurement

NR: male : 14-16.5 g/100mL or 14-16.5g% 140-165 g/L female: 12-15g/100mL

14

Used in counting RBC, WBC, Platelet, absolute eosinophil count, sperm count With thick cover slip Improved Neubauer Counting Chamber

hemacytometer

15

specific hemacytometer in the lab

Improved Neubauer Counting Chamber

16

# of WBC in 1 cu mm of blood Dil fluid must lyze RBC

WBC count

17

materials for WBC count

WBC pipette Dil fluid hemacytometer

18

procedure for wbc count

Aspirate blood 0.5 mark. Wipe sides Aspirate RBC dil fluid up to 101 mark. Mix dil’n 1:200 Discard 5-6 gtt Charge counting chamber Stand 5min Count RBC in HPO

19

materials for differential count

Blood 2 slides Differential stain Pasteur pipette Cedar wood oil

20

High refractive index, thereby increasing the numerical aperture of the objective lens Have specific optical and viscosity characteristics

cedar wood oil

21

describe cover glass used in differential count

thicker, less flexible, surface tension of the fluid will not deform them, height of the fluid is standardized

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procedure for differential count

Prepare smear Dry smear on flat surface Stain smear Count 100 cells in OIO

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technique used in differential count

strip differential technique

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how many WBC must be counted in a smear

100

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devices used for DIFFERENTIAL COUNT

hand tally counter differential counter

26

how many quadrants are there for WBC

4

27

What are counted in WBC or RBC

dots

28

how many secondary squares are in improved neubauer

9

29

how many tertiary squares are in 1 WBC square

16

30

what is the total number of tertiary squares of a WBC

16 x 4 = 64

31

1 RBC square = ___ tertiary squares

5

32

1 RBC tertiary square  =___ small squares

16

33

total number of small RBCsquares 

16 x 5 quadrants = 80 small squares

34

what are the staining solutions in differential count (list in order)

1st: ethanol

2nd: eosin

3rd: methylene blue

4th: buffer or distilled water

35

staining solution for fixing the slide

ethanol

36

staining solution for staining

eosin

methylene blue

37

staining solution for rinisng solution or stains

buffer or distilled water

38

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