Flashcards in CC Lab Deck (44):

0

## reading crea absorbance for A1 is done after

### 30secs

1

## formula for cholesterol in mg/dL

### 200 x (A sample / A STD)

4

## incubation for cholesterol

### 10mins @ 20-25 rm temp

5

## soln used in creatinine det.

### picric acid

7

## reading of absorbance for crea det for A2 is done

### after 2mins

8

## formula for creatinine conc. in mg/dL

### 2.0 x (delta sample / delta STD)

9

## waveleth at which creatinine complex is read

### 520nm

10

## enzymes used in cholesterol det

###
cholesteril esterase

cholesterol oxidase

peroxidase

11

## indicator in cholesterol det

### quinoneimine

12

## how many times is absorbamce read in crea det

### twice

13

## wavelength at which cholesteril is read

### 500nm

14

## formula for cholesterol in mmol/L

### 5.17 x (A sample / A STD)

17

## formula for crea det in umol/L

### 176.8 x (delta sample / delta STD)

24

## amount of working rgt in crea det

### 1mL

27

## Method for creatinine det.

### Jaffe's rxn

28

## wavelenth for HDLc

### 593nm

29

## formula for TG in mg/dL

### 200 x (A sample / A STD)

30

## formula for TG in mmol/L

### 2.28 x (A sample / A STD)

31

## Friedewald's formula

###
LDLc = TC - HDLc - TG/5 (mg/dL)

LDLc = TC - HDLc - TG/ 2.175 (mmol/L)

32

## indicator product of TG det

### quinoneimine

33

## enzyme for TG det

###
lipase, glycerol kinase

34

## protein not manufactured by liver

### immunoglobulins

35

## standards for proteins

### 8mg/dL or 80g/L

36

## incubation for TP det

### 10mins @ rm tmp

37

## wavelenth for TP det

### 546nm (520-580nm)

38

## rxn for TP det

### Biuret's rxn

39

## product of Biuret's

### violet colored compound

40

## standard for albumin

### 4g/dL or 40 g/L

41

## wavelength of albumin det

### 578nm

42

## index for assessing liver function

### albumin

43

## component Biuret's rgt

###
NaOH

potassium sodium tartrate

copper sulfate

ptassium iodide

44

## standard of TG

### 200mg/dL or 2.28 mmol/L

45

## standards for urea

###
80mg/dL

13.3 mmol/L

46

## how many times is urea test sample incubated

### twice

47

## first incubation of urea test is

### 5mins @ rm temp

48

## 2nd incubation for urea test is

### 10mins @ rm temp

49

## standards for serum BUN

###
37.28mg/dL

6.2 mmol/L

50

## complex formed at creatinine test

### creatinine-picrate complex

51

## functions to remove excess cholesterol

### HDLc

52

## amount of enzyme in HDLc test

### .75 mL

53

## amount of serum in crea test

### .1 mL

54

## amount of substrate in HDLc test

### .25 mL

55

## which is added first in HDLc test

### enzyme

56