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Flashcards in CC Lab Deck (44):
0

reading crea absorbance for A1 is done after

30secs

1

formula for cholesterol in mg/dL

200 x (A sample / A STD)

4

incubation for cholesterol

10mins @ 20-25 rm temp

5

soln used in creatinine det.

picric acid

7

reading of absorbance for crea det for A2 is done

after 2mins

8

formula for creatinine conc. in mg/dL

2.0 x (delta sample / delta STD)

9

waveleth at which creatinine complex is read

520nm

10

enzymes used in cholesterol det

cholesteril esterase
cholesterol oxidase
peroxidase

11

indicator in cholesterol det

quinoneimine

12

how many times is absorbamce read in crea det

twice

13

wavelength at which cholesteril is read

500nm

14

formula for cholesterol in mmol/L

5.17 x (A sample / A STD)

17

formula for crea det in umol/L

176.8 x (delta sample / delta STD)

24

amount of working rgt in crea det

1mL

27

Method for creatinine det.

Jaffe's rxn

28

wavelenth for HDLc

593nm

29

formula for TG in mg/dL

200 x (A sample / A STD)

30

formula for TG in mmol/L

2.28 x (A sample / A STD)

31

Friedewald's formula

LDLc = TC - HDLc - TG/5 (mg/dL)

LDLc = TC - HDLc - TG/ 2.175 (mmol/L)

32

indicator product of TG det

quinoneimine

33

enzyme for TG det

lipase, glycerol kinase

34

protein not manufactured by liver

immunoglobulins

35

standards for proteins

8mg/dL or 80g/L

36

incubation for TP det

10mins @ rm tmp

37

wavelenth for TP det

546nm (520-580nm)

38

rxn for TP det

Biuret's rxn

39

product of Biuret's

violet colored compound

40

standard for albumin

4g/dL or 40 g/L

41

wavelength of albumin det

578nm

42

index for assessing liver function

albumin

43

component Biuret's rgt

NaOH
potassium sodium tartrate
copper sulfate
ptassium iodide

44

standard of TG

200mg/dL or 2.28 mmol/L

45

standards for urea

80mg/dL
13.3 mmol/L

46

how many times is urea test sample incubated

twice

47

first incubation of urea test is

5mins @ rm temp

48

2nd incubation for urea test is

10mins @ rm temp

49

standards for serum BUN

37.28mg/dL
6.2 mmol/L

50

complex formed at creatinine test

creatinine-picrate complex

51

functions to remove excess cholesterol

HDLc

52

amount of enzyme in HDLc test

.75 mL

53

amount of serum in crea test

.1 mL

54

amount of substrate in HDLc test

.25 mL

55

which is added first in HDLc test

enzyme

56

incubation for enzyme in HDLc test

5mins @ 37C