CC Lab - Glucose Flashcards Preview

► Med Misc 05 > CC Lab - Glucose > Flashcards

Flashcards in CC Lab - Glucose Deck (40):
1

measures blood glucose exactly 2 hours after you start eating a meal

Two-hour Post Prandial Blood Sugar

2

how many carbs (in g) must be eaten for the Two-hour Post Prandial Blood Sugar

at least 100g

3

glycosylated hemoglobin

HbA1C

4

Other names of gluc

dextrose and blood sugar

5

possible tests requested by physician

FBS
RBS
OGTT, OGCT
2 PPBS
HbA1c

6

test for pregnant women/with gestational diabetes

OGTT/OGCT

7

test which requires NPO for 6-8 hrs

FBS

8

Test for monitoring DM

HbA1C

9

HbA1C test is done every ___

3 months

10

excess glucose is stored in __

liver

11

excess glucose may be stored as (2)

glycogen
adipose tissue

12

hormones that regulate gluc levels

insulin and glucagon

13

a hyperglycemic agent

glucagon

14

primary hypoglycemic agent

insulin

15

methods for glucose determination (3)

reduction method
schiff base reaction with orthotoluidine
enzymatic method

16

experiment proper (name??)

enzymatic colorimetric glucose testing

17

most important carbohydrate

glucose

18

CC which needs EDTA tube

HbA1c

19

the rest of CC tests need (Color of top)

red or yellow top

20

Principle in glucose testing (?)

glucose + oxygen + water ------> gluconic acid + H2O2

see notebook

21

insulin is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

gluconeogenesis

22

glucagon is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

glycogenolysis

23

3 methods for glucose determination

reduction method
schiff base reaction with orthotoluidine

24

3 methods for glucose determination
alkaline method
based on the ability of glucose to reduce copper or ferricyanide

reduction method

25

type of reduction method which includes Nelson-Somogyi, Folin-Wu, Iodometric

Copper reduction

26

type of reduction method
inverse colorimetry

Ferricyanide method

27

principle in ferricyanide method

ferricyanide (yellow) is converted to ferrocyanide (colorless)

28

3 methods for glucose determination
in a hot acidic medium or glacial acetic acid

schiff base reaction with orthotoluidine

29

Purpose of glucose measurement

Determine capacity of body to maintain normal level of glucose

30

glucose measurement significant in diagnosing what disease

Diabetes mellitus

31

Indicator in the glucose measurement

Quinoneimine dye

32

Color of quinoneimine dye

Red violet

33

Assay requirements

Wavelength - 500 nm
Optical path - 1 cm
Temperature - 20-25C
Measurement - against reagent blank

34

Only --- reagent blank used per series

1

35

How long to incubate and at what temp

10 min
20-25

36

How much standard solution must be placed

0.01 ml

37

How much control normal serum, control pathologic serum, patient's serum and standard solution must be placed

0.01 ml

38

Total volume in the cuvets

1.01 ml except for the blank which is 1.0 ml only

39

Pipetting scheme

Semi-micro

40

Purpose of glucose measurement

Determine capacity of body to maintain normal level of glucose

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