CC Lab - Spectrophotometry Flashcards Preview

► Med Misc 05 > CC Lab - Spectrophotometry > Flashcards

Flashcards in CC Lab - Spectrophotometry Deck (28):
1

major categories of analytical techniques

spectrometry
luminescence
electroanalytical method
chromatography

2

main principle of spectro

beer's law/beer-lambert's law

3

3 major methods for calculation of unknown solution

direct comparison/ratio of standard to unknown
use of standard curve
use of molar absorptivity value

4

source of reagent for exp

reagent kit

5

3 substances found in reagent

catalyst
enzyme
buffer

6

type of spectrophotometer in the lab

Humalyzer Primus

7

old spectrophotometer

ALS 2000

8

Conventional parts of spectrophotometer (6)

exciter lamp
variable slit diaphragm
monochromator
sample holder
photodetector
read out device

9

parts of spectrophotometer
light source

exciter lamp

10

parts of spectrophotometer
separates radiant energy

monochromator

11

parts of spectrophotometer
isolate narrow band of light

variable slit diaphragm

12

wavelength of glucose

500 nm

13

units of wavelength

nm or mu or 10^-9m

14

parts of spectrophotometer
holds smaple

sample holder/cuvetter

15

shape of cuvette

round or square

16

parts of spectrophotometer
reads amount of transmitted light then converts it to an electrical signal

photodetector

17

parts of spectrophotometer
galvanometer
converts electric signal to a readable data

read out device

18

keypad (4 commands)

ESC
Enter
Rinse
Paper

19

rinsing solution

triple distilled water

20

wait how many min after turning on the spectrophotometer

5 min

21

2 types of operating procedure

Kinetic or rate method

22

Operating procedure for ALS 2000 Spectrophotometer

Endpoint method

23

No sx
To set reading at 0

Blank

24

Blank solution with reagent

Reagent blank

25

Blank soln with only the sample, no rgt

Serum or sample blank

26

Empty test tube to set reading at zero

Air blank

27

Tool for precision which BEHAVES LIKE A SPECIMEN

Controls

28

2 types of operating procedure

Kinetic or rate method
Endpoint method

Decks in ► Med Misc 05 Class (295):