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Flashcards in CC LAB - TPAG Deck (18):
1


􏰀 Based on measurement of nitrogen content 􏰀

Kjeldahl Method

2

Reference method

Kjeldahl Method

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􏰀 Digesting agent of Kjeldahl Method

H2SO4

4

Most commonly used
􏰀

Biuret Method

5

Need two peptide bonds and an alkaline medium

Biuret Method

6

􏰀 Composition of Biuret Reagent:

a) Copper sulfate- provides the cupric ion that complexes with the peptide bonds
b) Potassium sodium tartrate (Rochelle’s salt)- prevents precipitation in alk solution
c) Potassium iodide- prevents autoreduction of copper

7

- important in maintaining plasma oncotic pressure

Albumin

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family of globular proteins that have higher molecular weights than albumin and are insoluble in pure water

Globulin-

9

Inverted A:G ratio means

presence of myeloma

10

Immediate response occurs with stress or inflammation caused by infection, injury or surgical trauma

Acute inflammatory response

11

• normal or ↓ albumin
• ↑ α1 and α2 globulins

Acute inflammatory response

12

Late response is correlated with chronic infection
(autoimmune diseases, chronic liver disease, chronic infection, cancer)

Chronic inflammatory response

13

• normal or ↓ albumin
•↑α1 or α2 globulins
•↑↑ γ globulins

Chronic inflammatory response

14

Cirrhosis can be caused by [2]

chronic alcohol abuse or viral hepatitis

15

↓ albumin
• ↓ α1, α2 and β globulins
• ↑ Ig A in γ-fraction

Liver damage - Cirrhosis

16

the kidney damage illustrates the long term loss of lower molecular weight proteins

Nephrotic syndrome

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retention of higher molecular weight proteins (↑↑ α2-macroglobulin and ↑β-globulin)

Nephrotic syndrome

18

↓ albumin and IgG – they are filtered in kidney)

Nephrotic syndrome

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