CC LEC 2 - Enzymes III Flashcards Preview

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Flashcards in CC LEC 2 - Enzymes III Deck (41):
1

Hydrolyzes the ester linkages of fats
(dietary triglycerides)alcohols & fatty acids

LPS

2

Major source of LPS

Pancreas

3

Isoenz of LPS

L1, L2, L3

4

most clinically significant &
sensitive LPS isoenz

L2

5

Detection almost only indicates acute pancreatitis

LPS

6

WHAT ENZ
Intraabdominal conditions:
 Penetrating duodenal ulcers  Perforatedpepticulcers
 Intestinal obstruction
 Acute cholecystitis

LPS

7

LPS activator

albumin/ionized calcium

8

LPS inhibitors

heavy metals (ex. quinine)

9

Substrate of turbidimetric and titrimetric LPS method

olive oil or triolein

10

LPS method
Simpler, more rapid
- Substrate: olive oil or triolein (more
used, pure form of triglycerides)

Turbidimetric Enzyme Reaction

11

LPS method
- Estimate liberated fatty acids
- Complicated because of lack of
stable uniform substrates
- Substrate: olive oil or triolein
- Indicator reaction:
Measures liberated fatty acids released by alkaline titration after 24 hours incubation

Cherry Crandall Method (Titrimetric)

12

Indicator of Cherry Crandall

phenolphthalein (salmon color)

13

indicator of sigma tiet

thymolphthalein

14

Oxidation of glucose-6-phosphate to
6- Phosphogluconate or corresponding
lactone

G6PD

15

Tissue sources:
 Adrenal cortex
 Spleen
 Thymus
 Lymph nodes
 Lactating mammary glands
 RBCs (little activity in normal serum)

G6PD

16

Maintain NADPH in reduced form

G6PD

17

? required to regenerate
sulfhydryl-containing proteins (ex.
glutathione) in its reduced state

NADPH

18

– protects
hemoglobin from oxidizing agents that may be present

Glutathione, reduced form

19

Deficiency is an inherited sex-linked trait & most common in African-Americans

G6PD

20

Only ? has different specimens depending on ↑/↓

G-6-PD

21

2 TYPES OF CHS

Pseudocholinesterase
True Cholinesterase

22

tissue sources type of CHS

 Serum
 Pancreas
 Liver (main)
 Heart
 White matter of CNS

23

type of CHS
Muscle relaxant during surgery

Pseudochs

24

type of CHS
Tissue sources:
 RBCs
 Nerve tissue/cells
 Brain

ACH/ True CHS

25

Hydrolyzes acetylcholine -> choline + acetic acid
For transmission of nerve impulses (acetylcholine is a major
neurotransmitter)

ACH/ True CHS

26

CHS methods

- Measures ↓ in pH resulting from the liberation of acetic acid
- Acid production

Michel Method (Electrometric Method)

27

CHS methods

- Measures the liberation of CO2 from the formation of acetic acid as acetylcholine is hydrolyzed

Manometric Method

28

CHS methods

- Most used
- Uses butylcholine (thiolester) as
substrate

Ellman Technique (Photometric Enzyme Reaction)

29

Hydrolysis of N-terminal residues from
certain peptides & amides containing free amino groups

Leucine aminopeptidase (LAP)

30

Tx sources
Urine, serum, bile

Leucine aminopeptidase (LAP)

31

Mtd for Leucine aminopeptidase (LAP)

Goldbarg and Rutenberg Method

32

Fluorochrome dyes for Goldbarg and Rutenberg Method

 Acridine orange
 Rhodamine
 FITC (fluorescein isothiocyanate)

33

Reversible conversion of ornithine to
citrulline (synthesis of urea)

Ornithine Carbamoyl Transferase (OCT)

34

OCT tissue source

liver

35

Mtds for OCT (2)

1. Reichard and Reichard Method
- Isotopic & microdiffusion technique
2. Colorimetric Method

36

Splitting of fructose-1,6-diphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone phosphate

Aldolase

37

Tissue sources:
 Widely distributed in all body cells
 Prominent in skeletal muscles, heart
muscles, liver (most), & RBCs

Aldolase

38

-Important in the breakdown of glucose  lactic acid (glycolytic breakdown)

Aldolase

39

Aldolase
Main tissue source: liver

B

40

Aldolase
Same tissue sources as previous, but
mainly found in the brain
(hippocampus, Purkinje cells)
- Catalyzes breakdown of alcohol

C

41

Aldolase
Found in muscles, RBCs, kidneys,
intestine, liver
- Most in liver
- Reacts with PLD2

A

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