CC LEC - Instrumentation Flashcards Preview

► Med Misc 05 > CC LEC - Instrumentation > Flashcards

Flashcards in CC LEC - Instrumentation Deck (18):
1

principle of spectrophotometry

measures array of lights or radiant energy absorbed or transmitted

2

state beer's law

Concentration of substance directly proportional to amount of light absorbed or logarithm of transmittedlight

3

component of spectro
source of light energy

exciter lamp

4

examples of light source

tungsten, halogen, vapor, mercury, xenon lamps

5

controls amount of light that enters monochrom

entrance slit

6

produces light of specific wavelength from exciter lamp

monochromator

7

types of monochromator

prism
diffraction gratings
interference fitlers

8

holds sample to be analyzed

cuvette or analytical cell

9

type of cuvette for acidic solution

soft glass

10

type of cuvette for alkaline solution

borosilicate

11

three types of cuvette

soft glass
borosilicate
quartz or plastics

12

detects and collects light energy transmitted
converts it into a current to produce a readable output

photodetector

13

types of photodetectors (4)

barrier layer cells
phototube
photomultiplier tubes
semiconductor

14

be used to protect cuvette from unwanted light that may cause measurement errors

light shield

15

preferably used in spectrophotometer because they reduce incident light

rectangular cuvette

16

use to check wavelength calibration of spectrophotometer

holmium oxide or didymium filters

17

filters or solutions with sharp cut off wavelength for transmission

nickel II sulfate

18

type of spectrophotometer
absorbance is measured at 2 different wavelengths using light from either single or 2 monochrom

double-beam

Decks in ► Med Misc 05 Class (295):