Cell Fractionation cont.

Question 1

Homogenation

Answer 1

Cells broken up by a homogeniser (blender). This release the organelles from the cell

Question 2

Ultracentrifugation

Answer 2

The process by which the fragments in the filtered homogenate are seperated in a machine called an ultracentrifuge

Question 3

Stages of ultracentrifugation

Answer 3

1. Tissue is cut up and kept in cold isotonic buffered solution
2. Cut up tissue further broken by a homogeniser
3. Homogenised tissue is spun in a ultracentrifuge at a low speed for 10 mins
4. Result of being spun in ultracentrifuge at low speed is that you get a supernatant and a sediment. you can spin it more times at faster speeds to get more sediments