Flashcards in Cellular Respiration Part 1 Deck (22):
Where does Glycolysis happen in the cell?
In the Cytoplasm
Does Glycolysis use oxygen?
What is overall happening in cellular respiration?
Glucose is being oxidized to Carbon Dioxide,
While Oxygen is being reduced to Water
What is the most universally prevalent process in cellular respiration that occurs in ALL organisms?
In step 1 of Glyolysis,
HEXOKINASE adds a phosphate group to Glucose in order to create Glucose 6 -P
1 ATP USED
In step 2 of Glycolysis.
Create an ISOMER of Glucose 6-P via
Glucose -6 -P is turned into Fructose -6 -P via an isomerase
In step 3 of Glycolisis,
PHOSPHORYLATE Fructose 6-P
to create P-Fructose-P (Fructose 1,6 Bisphosphate)
Enzyme Used: PHOSPHOFRUCTOKINASE
1 ATP used
In Step 4 of Glycolysis
Frcutose 1, 6 BisP is broken into two GAP (glyceraldehyde -3 -P molecules)
In Step 5 of Glycolysis
GAP is turned into PEP (phosphoenolpyruvate)
4 ATP GAINED
2 NADH GAINED
In Step 6 of Glycolysis
PEP is turned into Pyruvate via PYRUVATE KINASE
What are the regulatory point of glycolysis?
Glucose 6-P has negative feed back on hexokinase
ATP has negative regulation on Phophofructokinase
What is the point of no return for the glycolysis?
When Fructose 6 Phosphate turns into Fructose 1,6 Bisphosphatase
What are other fates of Glucose 6-P other than glycolysis?
1) Pentose Phosphate Pathway
2) It can be turned back into glucose and released into the bloodstream
Where does the PDC/Kreb's cycle take place?
The mitochondrial matrix
Do you need oxygen for PDC/Kreb's cycle?
Yes, you do use it, but it is used indirectly
What are the components of mitochondria
From outer most to innermost:
1) Outermost Membrane
2) Intermembrane space
3) Inner Mitochondrial membrane
4) Mitochondrial matrix
Where do the pyruvate from the cytoplasm go to in order to continue PDC/Kreb's Cycle?
They enter the matrix of the mitochondria
What are the overall reactants and products of of PDC step?
PDC Reactants, products:
Reactants: 2 pyruvate
Products : 2 Acetyl Co-A, 2NADH, 2CO2
What are the overall products and reactants for the Kreb's Cycle?
Kreb's Cycle Reactants and Products
Kreb's Cycle is not purely a Reactants vs. Products Equation, it is a cycle.
1) Oxaloacetate (4C) + Acetyl CoA (2C)
3) 6C-5C (+NADH, CO2 released)
4) 5C-4C ( +NADH, CO2 released)
5) 4C-4C (+GTP,. +NADH, +FADH2)
After the PDC/Kreb's cycle and Glycolysis, what is the overall numbers of NADH, FADH, GTP there are that will go into the Electron Transport chain and Oxidative Phosphorylation per Glucose molecule?
How much ATP does this constitute to?
There is a total of
From Glycolysis: 2 NADH
From PDC: 2 NADH gained
From Kreb's Cycle: 6 NADH, 2 FADH2
Total: 10 NADH, 2 FADH2 gained
2 NADH from Glycolysis: 3 ATP
8 NADH from PDC+ Kreb's Cycle: 20 ATP
2 FADH2 from Kreb's Cycle: `3 ATP
2 ATP from Glycolycis+ 2 GTP from Kreb's Cycle: 4 ATP
30 ATP total
What are the 5 Transmembrane proteins involved in the Electron transport chain?
1) NADH Dehydrogenase
2) CO Q
3) Cytochrome C
4) Cyt C oxidase
5) Lastly, there is a proton pump that couples H+ moving down gradient back to mitochondrial matrix with ATP generation
This is where the 10 NADH and 2 FADH2's "energy" is turned into 26 ATP