Flashcards in Ch 2: Studying Microbes Deck (19):
What are the 5 "I's" of the Microbiology lab?
1. Inoculation - specimen placed in artificial growth environtment
2. Incubation - growth
3. Isolation - separate species of interest
4. Inspection - Observe results
5. Identification - determine species identity
What is inoculation?
Creating a culture from a specimen
-flame sterilize inoculating loop (aseptic)
-Collect specimen, transfer to agar dish
-also can transfer to broth tube
What is incubation?
For each microbe, culture, requires time and temp
What is a culture medium?
Artificial growth environment
-Liquid media (broth) or solid media (agar)
What is a selective medium?
contains growth inhibitor that inhibits growth of many bacteria
What is a differential medium?
contains biochemical indicator that changes color to show an acid is produced during fermentation of a sugar
What is isolation?
A dilution technique used to separate microscopic cells
-grow macroscopic colonies (pure cultures)
What are the different methods of isolation?
Loop dilution and Streak Plate
What is inspection (part of the 5 I's)?
1) Microscopic appearance
2) Cellular metabolism and function
3) Genetic and Immunological characteristics
Which of the 5 I's uses the loop-dilution technique?
What are the components of a selective broth medium for the growth of staphylococci?
1. Protein rich nutrient extract
What is resolution?
measurement of the clarity of the image produced
-dependent on wavelength of light source
WHat is light microscopy?
Uses Glass lens that causes light refraction resulting in magnification
What are the parts of the light microscope?
Ocular Lens = what you look through
Objective lens = the different magnifications
Condenser lens = focuses light
Light source = bulb
How do you create contrast in cell structures?
Besides staining, how can contrast be achieved?
1) Fluorescent microscopy
2) Confocal microscopy
Which part of the microscope magnifies the image of the specimen?
Objective lens and Ocular lens
If you wanted to visualize a cross-section of a 20 nm virus particle, what type of microscopy would be used?
Transmission Electron Microscope (TEM)