Chapter 3: DNA Replication Flashcards Preview

Genetics > Chapter 3: DNA Replication > Flashcards

Flashcards in Chapter 3: DNA Replication Deck (18):
1

Semi-conservative??

- one DNA strand from parental helix acts as the template
- one newly synthesized strand

2

dNTPs are added to the _' end of the chain

3' OH

3

DNA is synthesized _' to _'

5' to 3'

4

The template for DNA synthesis must be single or double stranded?

- single!
- there must be a strand that extends on past the template

5

What are the primary replication enzymes?

Poly I and Poly III

6

there are __ nt/s in bacteria and _-_ nt/s in humans?

- 850
- 60-90

7

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaA?

- initiator proteins bind.
L> leads to the denaturation of DNA
L> binds at 9-bp

8

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaB ?

- helicase....binds to DNA with the help of DnaC
L> break H-bonds btwn bases
L>r 13-bp since its rich in A and T

9

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaG???

primase binds....its a DnaG gene product.... DNA polymerase binds

10

What are the three core enzymes of DNA Poly III?

- DNA E
DNA Q
HOLE

11

What are the three enzymes of DNA poly I?

- DNA E , DNA Q , DA e

12

Replication fork:
- The double stranded DNA section __ to reveal __ template strands

- opens
ssDNA

13

Replication fork
- Leading strand?

- 5-3 strand synthesized continuously

14

Replication fork
lagging strand?

- 3-5' strand synthesized discontinuously

15

Eukaryotic Polymerases?
- Pol aplha/primase?

- initiates new strands in replication via primase
L> Poly a adds 10-20 nt of DNA

16

Eukaryotic Polymerases?
-Pol e - epsilon?

- synthesize the leading strand

17

Eukaryotic Polymerases?
- Poly delta?

- synthesizes the lagging strand.

18

Eukaryotic Replicons
- More than 1 ___ per chromosome.

- replication
- bidirectional at each origin.