Clearing

Question 1

Ideal amount

Answer 1

10x

Question 2

Used when the tossue is cleared directly from water (frozen section)

Answer 2

Glycerin/Gum syrup

Question 3

Excellent and true clearing agent; most rapid; can be used with celloidin sections

Answer 3

Xylene

Question 4

Substitute to xylene or benzene; nit carcinogenic; slower than benzene; expensive

Answer 4

Toluene

Question 5

Rapid actung; for urgent biopsies and routine pruposes; higly flammable; carcinogenic; damage bone marrow causing aplastic anemia

Answer 5

Benzene

Question 6

Best of the traditional clearing agents for routine use; slower than xylene but less brittleness; widest latitude; for tough and large specimens; best for delicate and higly cellular specimens; does not make tissues transparent; hepatotoxic

Answer 6

Chloroform

Question 7

Clears both paraffin and celloidin sections; for CNS and cytological studies of smooth muscle and skin; very expensive (2 changes only); quality is not uniform; extremely slow; milky

Answer 7

Cedarwood oil

Question 8

For clearing embryos, insects, and very delicate specimens

Answer 8

Aniline oil

Question 9

Minimum shrinkage, removes aniline dyes; dissolves celloidin; tissues become brittle; tendency to be adukterated; expensive

Answer 9

Clove oil

Question 10

Similar to chloroform but cheaper; toxic

Answer 10

Carbon tetrachloride/CCl4

Question 11

Dehydrates and clears at the same time

Answer 11

Tetrahydrofuran

Question 12

Slow acting; used for double embedding trchniques

Answer 12

Methyl benzoate; Methyl salicylate