CM LEC - CSF up to CSF Protein Flashcards Preview

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Flashcards in CM LEC - CSF up to CSF Protein Deck (136):
1

CSF produced by

choroid plexus

2

choroid plexus produces how many percent of CSF

70%

3

where does the 30% CSF come from

plasma filtration

4

provides nutrients to CNS

CSF

5

channel for removal of waste products

CSF

6

mechanical buffer

CSF

7

protects brain and spinal cord from sudden pressure changes

CSF

8

CSF is also called __

fluid under pressure

9

volume regulator

CSF

10

normal CSF volume baby

10-60 ml

11

normal CSF volume adult

140 - 170 ml

12

rate of formation

20 ml CSF/hour

13

gold std for diagnosis of meningitis

CSF analysis

14

3 layers of meninges

dura
arachnoid
pia mater

15

types of inflammation

septic
aseptic

16

type of inflammation
viral, bacterial cause or other infectious agents

septic

17

types of inflammation
due to trauma

aseptic

18

methods of CSF collection

lumbar, cisternal, ventricular puncture

19

methods of CSF collection
w/in 3rd, 4th, 5th lumbar vertebrae

lumbar puncture
spinal puncture
lumbar tap

20

methods of CSF collection
most common

lumbar puncture
spinal puncture
lumbar tap

21

methods of CSF collection
used among infants only
done while baby's fontanelle is open

ventricular puncture

22

methods of CSF collection
at base of the skull, not popular

cisternal

23

too much sx can't be collected unless the pressure is

normal

24

opening pressure for adult

90-180 mm H20

25

opening pressure for young

10 - 100 mm H20

26

2 materials mentioned, used for csf collection

L-shaped adaptor (where needle is attached)
sterile manometer tube (measures pressure)

27

when can doctor aspirate?

when pressure is constant

28

closing pressure

45 -90 mm H20

29

when pressure suddenly drops

subarachnoid blockage

30

when pressure suddenly drops, do not ___

aspirate

31

determines the subarachnoid block which lowers the opening pressure

Queckenstendt test

32

CSF is always considered a STAT specimen except for vial # ?

2

33

vial # 1 is for what section

Chemistry/Serology

34

vial # 2 is for what section

Bacteriology

35

vial # 3 is for what section

Hematology

36

at what temp should vial # 1 be kept

freezing temp/freezer

37

at what temp should vial # 2 be kept

RT/incubator

38

at what temp should vial # 3 be kept

refrigerator temp

39

routine tests done on csf in chem section

CSF glucose
CSF protein

40

special tests done on csf in chem section

CSF CK - MB
CSF CK -MT
CSF LDH
Glutamine
Lactate

41

tests done on csf in serology section

vDRL
FTA - ABS
CSF C-RP

42

vDRL meaning

veneral disease research laboratory

43

FTA-ABS meaning

Fluorescent Treponemal Antibody Absorption Test

44

tests done on csf in bacte section

G/S
C/S
AFB
India Ink

45

tests done on csf in hema section

WBC Count
WBC Differential Count

46

RBC in CSF indication of

traumatic puncture

47

physical analysis is done on w/c section

hema

48

chemical analysis is done on w/c section

cc

49

microscopic analysis is done on w/c section

bacte

50

possible appearance of CSF (5)

crystal clear
cloudy, milky, turbid
xanthochromic
clotted
pellicle

51

normal appearance of csf

colorless and clear

52

CSF comparable to _

water

53

blank used for CSF analysis

distilled water

54

appearance of CSF
indicates no cell count

clear

55

appearance of CSF
indicates moderate cell count

cloudy/hazy

56

appearance of CSF
indicates high cell count

turbid

57

purpose of dilution of sx prior to counting

so cells are not overlapping

58

appearance of CSF
pale orange/ reddish/ yellowish discoloration

xanthochromic

59

xanthochromic sx has the presence of (4)

bilirubin, carotene, Hgb, protein

60

appearance of CSF
indicates traumatic puncture

clotted

61

appearance of CSF
tiny white aggregates on sample surface

pellicle

62

pellicle also described as __

cobweb or tigerweb

63

appearance of CSF
correlated with TB meningitis

pellicle

64

specimen suspected with __ and __ should not be kept in fridge

H. influenzae and N. meningitis

65

specimen suspected with H. influenzae and N. meningitis
because these are __

labile, killed at certain temp

66

Bloody specimen can have 2 causes

Traumatic aspiration
True intracranial bleeding

67

distribution of RBCs in Traumatic aspiration

uneven

68

distribution of RBCs in True intracranial bleeding

even

69

Presence of clot in TA

+ due to plasma contamination

70

Presence of clot in TIB

-

71

Xanthochromia in TA

clear

72

Xanthochromia in TIB

xanthochromic

73

CSF CK-BB in TA

not mentioned

74

CSF CK-BB in TIB

higher

75

Isoenzymes of CK

CK - MM
CK - BB
CK - MB

76

CSF Lactate in TA

not mentioned

77

CSF Lactate in TIB

increased

78

when does lactate increase

during tissue destruction

79

transparency of csf

clear

80

sp gr of csf

1.006 - 1.008

81

pH of csf

7.30 - 7.35

82

WBC Count Normal Values for adult

0-5WBC/ ul

83

WBC Count Normal Values for infants

0-30 WBC/ ul

84

increased WBC in CSF

pleocytosis

85

if sx is clear
what is the dilution
manner of counting
where to count/ what formula to use

no dilution
direct counting
count in 9 large squares

86

if sx is hazy
what is the dilution
manner of counting
where to count/ what formula to use

1:10 dilution
count in 5 large squares
use std formula for actual wbc count

87

if sx is turbid
what is the dilution
manner of counting
where to count/ what formula to use

1:20
count in 4 large squares
use std formula for actual wbc count

88

diluting fluid for CSF WBC count

Tuerk's
3% Acetic Acid

89

what tube is used in charging for CSF WBC count

plain blue capillary tube

90

manner of mixing for CSF WBC count

inversion

91

for CSF WBC count, may be counted as a wbc

nucleated red cell

92

methods of obtaining sediment for diff count

sedimentation
filtration
cytocentrifugation
centrifugation

93

most common, most backward method of obtaining sediment for diff count

sedimentation

94

what to d with supernatant after sedimentation

give to chem section

95

stain used in CSF diff count

wright stain

96

advanced method of obtaining sediment for diff count

cytocentrifugation

97

in diff count, if cells counted do not reach 100, report only the ___ seen

number of cell types seen

98

method of obtaining sediment for diff count w/ good cell recovery

cytocentrifugation

99

method of obtaining sediment for diff count w/ complete cell recovery

filtration

100

stain for blood cells

wright

101

stain for cancer cells

papanicolaou

102

stain for parasitic cells

giemsa

103

CSF Cell constituents and %

70% lympho
30% mono

104

> 70% PMN indicates

bacteria meningitis

105

Early viral, fungal or tubercular meningitis demonstrates increase in what cells

PMN

106

Chronic viral, fungal or tubercular meningitis demonstrates increase in what cells

Lympho

107

parasitic meningitis demonstrates increase in what cells

eosino

108

bacterial meningitis demonstrates increase in what cells

PMN

109

Immature leukocytes indicates

leukemia

110

malignant cells indicates

tumor/cancer

111

plasma cells and reactive lympho indicates

HIV/ AIDS

112

macrophages in brain

microglial cells

113

bacteria that causes increase in lympho

Listeria monocytogenes

114

fungi with star burst appearance

C. neoformans

115

N.V. of CSF protein

15 - 45 mg/dL

116

Elevated CSF protein indicates

damage in BBB

117

why CSF protein is

CSF protein is a product of filtration, proteins cannot pass the filtering membrane

118

conditions of increased CSF protein

meningitis
traumatic tap
IgG synthesis

119

in traumatic tap, a drop of plasma causes how much increase of protein

10x

120

globulin produced during infection

IgG

121

conditions of decreased CSF protein

Elevated intracranial pressure
large sample removal
hyperthyroidism
otorrhea

122

CSF leaks from brain to ears

otorrhea

123

CSF leaks from brain to nose

rhinorrhea

124

protein fraction normally in csf (decreasing order)

Alb
Pre-alb
Ceruloplasmin
Transferrin
IgA
IgG

125

Marker to differentiate CSF from other body fluids

Tau protein or T protein or carbohydrate deficient protein (?)

126

proteins not normally in CSF

fibrinogen
IgM
beta lipoprotein

127

precipitating agents (2)

SSA
TCA

128

SSA meaning

sulfosalicylic acid

129

TCA meaning

trichloroacetic acid

130

can ppt alb fraction better than glob fraction

SSA

131

can ppt alb fraction and glob fraction better

TCA

132

more sensitive ppt agent

TCA

133

Supernatant (Alb) measured using what dyes

BCG
MO
HABA

134

Used to visualize all protein fractions

electrophoresis

135

type of electrophoresis commonly used for CSF

PAGE

136

first choice dye
has affinity for all protein fractions

Coomassie Brilliant Blue

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