Collection and Handling of Biological Samples Flashcards Preview

RUSVM Clinical Pathology > Collection and Handling of Biological Samples > Flashcards

Flashcards in Collection and Handling of Biological Samples Deck (9):
1

Blood for biochemical profile should be analyzed in what time frame?

Blood should be allowed to clot for 15-30 minutes

Serum should be separated by centrifugation

Refrigerate serum or freeze if not being analyzed within 2 days

2

Green Top

Heparin

Anticoagulant - inhibits thrombin

Contains fibrinogen

Uses: Chemistry, Avian/reptile CBC and chem, Plasma colloid oncotic pressure, measurement of electrolytes, specific tests

3

Tiger Top (Red and Black)

Gel that promotes blood clot formation and separates cells from serum

Used for chemistry and serology

4

Blue Top

Citrate

Anti-Coagulant, Ca Chelator

Use: Coag tests, PIVKA, Antithrombin, Coag Factor Analysis, Von Willebrands Factor Analysis

5

Gray Top

Sodium Fluoride Oxalate

Anti-coagulant, Ca chelator

Inhibits glucose metabolism

Use: serial glucose, lactate and pyruvate

6

Red Top Tube

No Anti-Coagulant

Blood is expected to clot

Serum used for biochemical profile and other tests

7

Lavender Top

EDTA to K Salt

Anticoagulant and Ca2+ chelator

Preserves cell morphology

Uses: CBC, Fibrinogen, Reticulocyte count, Buffy coat analysis, fluid analysis, blood banks, coombs test, PCR, endogenous ACTH

8

Blood for a CBC should be analyzed in what time frame

Within the hour of taking the sample

9

Volume of blood needed for CBC and Biochemical Profile?

5 mL