Complement Cascade Flashcards Preview

IS > Complement Cascade > Flashcards

Flashcards in Complement Cascade Deck (53):
1

Decay accelerating factor deficiency

Paroxysmal nocturnal hemoglobinuria

3

Originally named for the protein properdin

Alternative pathway

5

Complement from 1 sp will react w/ immunoglobulin of another sp from the same taxonomic order

Non-specific serologic reagent

7

The final stage of the complement cascade

Cytolysis

8

Coating of antigen w/ specific complement peptide by specificreceptor on phagocytes for ingestion

Opsonization

9

Complement towards immune response

Immunologic response

10

Complement activity is destroyed when ...

Heating sera at 56C for 30minutes

11

It can provide a level of immunity even in the absence of antibody

Complement

12

C2 deficiency

Atherosclerosis

13

Fragment that loses activity as a result of further proteolysis

iC3b

14

Does complement icreases after inflammation?

No

15

MASP - ...

Mannose-binding lectin associate serine protease

16

Generate enzyme activity to begin the cascade

C1rs

17

Recognition stage in classical pathway is composed of

C1 complex (c1qrs)

18

Polymerizes to cause lysis

C9

19

C4b if not bind, will react to H2O molecule forming

iC4b

21

C4b is combined with ...

Magnesium ions

22

A serum enzyme that inactivates C3b and C4b

Factor I

23

C3 convertase (alternative)

C3bBb

26

Factor I/H deficiency

Recurrent pyogenic infection

27

C5 convertase (typical)

C4b2a3b

28

C5 convertase (alternative)

C3bBb3bP

29

Serves as the pivotal point for all 3 pathways

C3

30

It is extremely labile and rapidly inactivated unless binds to a glycoprotein

C5b

31

Nesseria infection

Properdin
MBL
C5 - C8

32

Has the high affinity for lipid constituents for the cell membrane

C5b67

34

Fluid-phase regulatory proteins

C1 inhibitor
Factor I
Factor H
S Protein
Factor J

37

Also known as vitronectin
Inhibits MAC in the cell membrane

S Protein

38

A plasma alpha2-globulin that inhibits activation at the first stages of both classical/lectin pathway

C1 inhibitor

39

Inhibits C1 complex formation
Inhibits cleavage of C3 by Bb

Factor J

40

Starts pore formation in the membrane

C8

41

Initiates membrane attack complex

C5

42

Binds C3b and C4b to enhance degradation of factor I

CR1 (CD35)

43

Most efficient cofactor of Factor I. Mediated cleavage of C3b

Membrane cofactor protein (CD46)

44

Capable of dissociating both classical and alternative pathway C3 convertase

Decay-Accelerating Factor

45

Factors that destroy complement in vitro

Anticogaulant - chelators
Heating
Normal serum inhibitor
Storage

46

Heating conditions

56C for 30mins destroys C1 & C2

50C for 20mins inactivates properdin and Factor B

47

Routine screening test for complement activity

Hemolytic assay
CH50 total hemolytic vomplement assay

48

CH50 total hemolytic complement assay

Hemolysis of sheep rbc sentisized with anti-sheep hemolysin measured in spectrophotometer

49

Sensitive and precise methods for measuring complement

Functional assay

50

Disasvantages for functional assay

More complex
Time-consuming
Require highly purified reagents
Costly

51

Antigenic assay that is highly specific and require fewer specialized reagents and considerably less time

Immunoassays

52

Lupus-like syndrome

C1
C4

53

Cell-associated regulatory proteins

Cr1 (CD35)
Membrane cofactor protein (CD46)
Decay-accelerating factor (CD55)

54

C1 INH deficiency

Hereditary angioedema

55

C3 deficiency

Glumerulonephritis

56

Pneumococcal diseases

MASP-2
MBL

57

The recognition phase starts once ...

C1s activates

59

MASP utilizes serum protein present at about ...

1.5 ug/ml

69

C3 convertase (classical/Lectin)

C4b2a

77

Refers to a group of glycoproteins that promotes inflammation and serves important role in host defense

Complement system

78

Binds to C3b to prevent binding of factor B and cofactors factor I to breakdown C3b

Factor H

100

He said it complements the action of antibody in destroying microorganism

Paul ehrlich